去甲二氢愈创木酸对内皮细胞影响的形态学观察

目的：探讨去甲二氢愈创木酸（NDGA）对内皮细胞形态的影响及特点。方法：采用倒置显微镜、光镜、电镜和流式细胞仪观察分析不同浓度NDGA作用下人脐静脉内皮细胞系ECV－304细胞形态和细胞调亡峰的变化。结果：①不同浓度（50－200μmol/L）的NDGA处理ECV－304细胞24－72h后或一定浓度（100μmol/L）的NDGA处理ECV－304细胞不同时间（1－7d）后，内皮细胞由多角形变长，呈梭形，细胞稀疏，核分裂明显减少，显示细胞增殖的抑制，上述变化与NDGA的浓度和作用时间有关；②NDGA处理后，部分细胞呈现出调亡样形态学改变，以48h为明显。流式细胞仪检测也出现明显高于对照的调亡峰即亚G0峰，12h时相点最高（20.42％）。此外，处理后的细胞也有变性、坏死等病理变化，与NDGA的浓度呈正比。结论：NDGA对内皮细胞的生长和增殖有抑制，形态学初步证明也有诱导调亡等作用，这在影响血管生成中可能具有一定意义。

Effect of nordihydroguaiaretic acid on the morphological changes of endothelial cells in vitro

Objective　To investigate morphological changes of endothelial cells after nordihydroguaiaretic acid (NDGA) treatment in vitro. Methods　The morphological changes of human umbilical vein endothelial cells (HUVEC) cell line ECV-304 and the cell apoptosis rate in sub-G0 phase were observed with invert, light and electron microscope and flow cytometry after NDGA treatment at different concentrations or with PBS (0.01 mol/L) as control. Results　①After the treatment of NDGA at 50～200 μmol/L for 1～3 d or up to 8 d at 100 μmol/L, ECV-304 cells tended to elongate into a shuttle-like sparse appearance and those in mitosis were decreased, indicating the suppression of cell proliferation. All these alteration was in a time-and dose-dependent manner. ②NDGA-treated ECV-304 cells displayed morphological features of apoptosis, especially at the 48th h after the treatment. With flow cytometry, the cells in sub-G0 phase were significantly increased, and reached its peak at hour 12 (20.42%) after NDGA treatment. In addition, the degeneration and necrosis of ECV-304 cells were related to the concentrations of NDGA. Conclusion　NDGA can inhibit the proliferation and growth of endothhelial cells, and induce apopotosis, which might also inhibit angiogenesis.