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去甲二氢愈创木酸对内皮细胞影响的形态学观察
引用本文:孙慧勤,陈兆珍,陈意生,史景泉,卞修武,辛榕. 去甲二氢愈创木酸对内皮细胞影响的形态学观察[J]. 第三军医大学学报, 2001, 23(3): 280-283
作者姓名:孙慧勤  陈兆珍  陈意生  史景泉  卞修武  辛榕
作者单位:1. 第三军医大学附属西南医院病理学研究所
2. 第三军医大学高原军事医学系中心实验室
基金项目:国家自然科学基金资助项目 !(3 9570 2 95),重庆市科委应用基础研究资助项目
摘    要:目的:探讨去甲二氢愈创木酸(NDGA)对内皮细胞形态的影响及特点。方法:采用倒置显微镜、光镜、电镜和流式细胞仪观察分析不同浓度NDGA作用下人脐静脉内皮细胞系ECV-304细胞形态和细胞调亡峰的变化。结果:①不同浓度(50-200μmol/L)的NDGA处理ECV-304细胞24-72h后或一定浓度(100μmol/L)的NDGA处理ECV-304细胞不同时间(1-7d)后,内皮细胞由多角形变长,呈梭形,细胞稀疏,核分裂明显减少,显示细胞增殖的抑制,上述变化与NDGA的浓度和作用时间有关;②NDGA处理后,部分细胞呈现出调亡样形态学改变,以48h为明显。流式细胞仪检测也出现明显高于对照的调亡峰即亚G0峰,12h时相点最高(20.42%)。此外,处理后的细胞也有变性、坏死等病理变化,与NDGA的浓度呈正比。结论:NDGA对内皮细胞的生长和增殖有抑制,形态学初步证明也有诱导调亡等作用,这在影响血管生成中可能具有一定意义。

关 键 词:去甲二氢愈创木酸 内皮细胞 细胞凋亡 形态学 血管生成
文章编号:1000-5404(2001)03-0280-04
修稿时间:2001-01-17

Effect of nordihydroguaiaretic acid on the morphological changes of endothelial cells in vitro
SUN Hui-qin,CHEN Zhao-zheng,CHEN Yi-sheng,SHI Jing-quan,BIAN Xiu-wu,XIN Rong. Effect of nordihydroguaiaretic acid on the morphological changes of endothelial cells in vitro[J]. Acta Academiae Medicinae Militaris Tertiae, 2001, 23(3): 280-283
Authors:SUN Hui-qin  CHEN Zhao-zheng  CHEN Yi-sheng  SHI Jing-quan  BIAN Xiu-wu  XIN Rong
Abstract:Objective To investigate morphological changes of endothelial cells after nordihydroguaiaretic acid (NDGA) treatment in vitro. Methods The morphological changes of human umbilical vein endothelial cells (HUVEC) cell line ECV-304 and the cell apoptosis rate in sub-G0 phase were observed with invert, light and electron microscope and flow cytometry after NDGA treatment at different concentrations or with PBS (0.01 mol/L) as control. Results ①After the treatment of NDGA at 50~200 μmol/L for 1~3 d or up to 8 d at 100 μmol/L, ECV-304 cells tended to elongate into a shuttle-like sparse appearance and those in mitosis were decreased, indicating the suppression of cell proliferation. All these alteration was in a time-and dose-dependent manner. ②NDGA-treated ECV-304 cells displayed morphological features of apoptosis, especially at the 48th h after the treatment. With flow cytometry, the cells in sub-G0 phase were significantly increased, and reached its peak at hour 12 (20.42%) after NDGA treatment. In addition, the degeneration and necrosis of ECV-304 cells were related to the concentrations of NDGA. Conclusion NDGA can inhibit the proliferation and growth of endothhelial cells, and induce apopotosis, which might also inhibit angiogenesis.
Keywords:nordihydroguaiaretic acid  endothelial cells  morphology  apopotosis
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