| Nod1受体激活对腹膜间皮细胞自噬相关蛋白表达的影响 |
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| 引用本文： | 伍军,邓行江,张魁正,何建发,何敏,黄洁平.Nod1受体激活对腹膜间皮细胞自噬相关蛋白表达的影响[J].中国现代医药杂志,2012,14(4):6-8. |
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| 作者姓名： | 伍军 邓行江 张魁正 何建发 何敏 黄洁平 |
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| 作者单位： | 512025,汕头大学医学院附属粤北人民医院肾内科 |
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| 基金项目： | 2011年广东省医学科学技术研究基金资助(青年项目,编号B2011315) |
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| 摘 要： | 目的探讨Nod1受体激活对腹膜间皮细胞自噬的影响。方法 Nod1受体激动剂iE-DAP作用大鼠原代培养腹膜间皮细胞0、1、2h,Western blot检测微管相关蛋白1轻链3(LC3)、Beclin-1的表达。结果随刺激时间延长,LC3-Ⅱ/LC3-Ⅰ蛋白表达比值增加,Beclin-1蛋白表达亦增加。结论 Nod1受体激活可诱导腹膜间皮细胞自噬的发生,这有可能发挥清除细胞内细菌感染的作用。
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| 关 键 词： | Nod1 腹膜间皮细胞 自噬 LC3 |
Nod1-agonist induced autophagy-related protein expression in rats peritoneal mesothelial cells |
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| Institution: | Wu Jun,Deng Xingjiang, Zhang Kuizheng,et al. Department of Nephrology,Yuebei People’s Hospital,the Affiliated of Shantou University Medical College,Shaoguan 512025 |
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| Abstract: | Objective To investigate the Nod1-agonist-induced autophagy-related protein expression in rats peritoneal mesothelial cells. Methods Rat peritoneal mesothelial cells in the logarithmic growth phase were cultured. Cells were treated with iE-DAP(Nod1-agonist)at 0h,1h and 2h. Western blot analysis were used to detect the expressions of microtubule-associated protein 1 light chain 3(LC3)and Beclin-1 in cell. Results Compared with the control group,the ratio of LC3-Ⅱ/LC3-Ⅰ and Beclin-1 protein expression increased with the prolongation of iE-DAP stimulation. Conclusion Autophagy of rat peritoneal mesothelial cells can be induced by Nod1-agonist, which may enhance the antibacterial capacity. |
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| Keywords: | Nod1 Peritoneal mesothelial cells Autophagy LC3 |
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