结核病患者痰样本培养前间期与培养阳性率的相关性分析

目的探讨结核病患者痰样本进行培养前间期和痰样本数量与培养阳性率的关系。方法在3个地市的20个区县6月内同时连续纳入涂阳肺结核病人,患者的痰样本运输到相应地市专科医院进行中和法分离培养,比较阳性率的差异。结果痰样本进行分离培养前的时间间隔与痰培养的阳性率呈负相关,从样本接收到分离培养间隔在7d内的痰样本培养阳性率高于大于7d的痰样本培养阳性率,差异具有统计学意义(P<0.05);样本运输时间间隔在3d内与大于3d的培养结果差异有统计学差异(P<0.05);结核病人留取痰样本的数量与培养的结果呈正相关,1份痰样本和2份痰样本以上的病人痰培养结果差异有统计学意义(P<0.001)。结论对可疑结核病患者进行痰分离培养时,需要留取2份以上痰样本,自留取痰液始,1周内完成分离培养操作具有可接受的培养阳性率。

Study of the correlation between pre-culture interval of sputum from TB patients and culture positive rate

Objective To learn the relationship between pre-culture interval of sputum from TB patients and the number of sputum with culture positive rate.Methods20 counties in 3 cities continuously recruited smear positive TB patients within 6 months.All the sputum was sent to TB laboratory of city hospitals for culture.Culture positive rate was analyzed.ResultsThere was a significant difference between pre-culture interval and culture positive rate(P<0.05).The sputum referral interval played a significant role on the culture positive rate(P<0.05).The number of sputum of TB patients was significantly related to culture positive rate(P<0.001).ConclusionsTB patient should produce at least 2 sputum and referral to hospital for culture within 3days.The culture should be done no more than 7 days since the day sputum produced.