HPLC测定小柴胡颗粒中柴胡皂苷、黄芩苷及甘草酸的含量

目的:建立以高效液相色谱法测定小柴胡颗粒中柴胡皂苷、黄芩苷和甘草酸含量的方法。方法:色谱柱为Kromasil C_(18),流动相为水(0.025%甲酸)-甲醇(0.025%甲酸),检测波长为250 nm。结果:柴胡皂苷在0.201 6μg/mL¹23.7μg/mL范围内线性关系良好(r=0.999 0),平均加样回收率为94.1%,RSD为1.62%(n=6)。黄芩苷在0.749μg/mL123.7μg/mL范围内线性关系良好(r=0.999 0),平均加样回收率为94.1%,RSD为1.62%(n=6)。黄芩苷在0.749μg/mL⁴58μg/mL范围内线性关系良好(r=0.999 5),平均加样回收率为95.2%,RSD为1.52%(n=6)。甘草酸在2.270μg/mL458μg/mL范围内线性关系良好(r=0.999 5),平均加样回收率为95.2%,RSD为1.52%(n=6)。甘草酸在2.270μg/mL¹35.5μg/mL范围内线性关系良好(r=0.999 5),平均加样回收率为94.8%,RSD为1.36%(n=6)。结论:该方法简便、灵敏、准确,可用于小柴胡颗粒的质量控制。

Determination of Saikosaponin, Baicalin and Glycyrrhizic Acid Content in Xiaochaihu Granule by HPLC

Objective ：To establish a method for determination of saikosaponin,baicalin and glycyrrhizic acid content in Xiaoehaihu granules. Method： HPLC was performed on Kromasil C18 column with water （0.025% methanol）-earbinol （0.025% methanol）as mobile phase,detection wavelength was set at 250 nm. Result：The linear range of saikosaponin was 0.201 6 μg/mL~123.7 μg/mL （r=0.999 0）,average recovery rate was 94.1%,RSD was 1.62%（n=6）. The linear range of baicalin was 0.749 μg/mL-458 μg/mL （r=0.999 5）,average recovery rate was 95.2%,RSD was 1.52%（n=6）. The linear range of glyeyrrhizie acid was 2.270 μg/mL-135.5 μg/mL （r=0.999 5）, average recovery rate was 94.8% ,RSD was 1.36% （ n=6 ）. Conclusion ： This method is simple, sensitive, accurate, and can be used for quality control of Xiaoehaihu granules.