siRNA下调表达HDGF对HepG2细胞增殖能力及ADAM9表达的影响

目的：探讨HDGF基因特异性siRNA对肝癌细胞系HepG2的增殖能力及ADAM9基因表达的影响.方法：设计并合成HDGF基因特异性的siRNA干扰序列,瞬时转染HepG2细胞.实时荧光定量PCR检测HDGF和ADAM9 mRNA的表达变化;MTT法观察细胞增殖能力的变化.结果：转染48h时HDGF和ADAM9的基因表达抑制最为明显,分别为阴性对照组表达量的7％和5％（P＜0.05）.转染48h时HepG2细胞增殖能力受到明显抑制（P＜0.05）.结论：HDGF siRNA能够显著抑制HDGF和ADAM9基因的表达,降低HepG2细胞的增殖能力.

Effects of down regulation HDGF by siRNA on proliferation of HepG2 cells and expression of ADAM9

Objective： To study the effect of siRNA targeting hepatoma - derived growth factor （HDGF） on prolif- eration of the hepatoma cell line HepG2 and expression of metalloprotease 9 （ADAM9）. Methods： Specific siRNA for HDGF gene was designed and synthesized, followed by transient transfection into the HepG2 cells. RT- PCR was used to examine the expression of HDGF and ADAM9. The proliferation of the HepG2 cells was examined by MTT assay. Results ：The expression of HDGF and ADAM9 was found to be significantly suppressed at 48 hour （decreased to 7% and 5% respectively, P 〈 O. 05 ）, and the proliferation of the HepG2 cells was also suppressed significantly at 48 hour （P 〈 0.05 ）. The expression of ADAM9 was positively correlated with the expression of HDGF, significantly sup- pressed at 48 hour. Conclusion： HDGF siRNA can significantly silence the expression of HDGF and ADAM9,inhibit the proliferation ability of the HepG2 cells.