| 储存磷光质屏放射自显影在测定兔血浆中^125I—重组人尿激酶原浓度的应用 |
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| 引用本文： | 袁守军,汤仲明,刘秀文,窦桂芳,宋海峰.储存磷光质屏放射自显影在测定兔血浆中^125I—重组人尿激酶原浓度的应用[J].解放军药学学报,2001,17(1):19-21,57,58. |
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| 作者姓名： | 袁守军 汤仲明 刘秀文 窦桂芳 宋海峰 |
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| 作者单位： | 中国人民解放军军事医学科学院放射医学研究所, |
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| 基金项目： | 国家自然科学基金!No .3 993 0 180 |
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| 摘 要： | 目的 探讨储存磷光质屏放射自显影结合聚丙烯酰胺凝胶电泳测定兔血中12 5I 重组人尿激酶原 (12 5I rhpro UK)浓度的方法。方法 将含12 5I rhpro UK样品的聚丙烯酰胺凝胶贴于储存磷光质屏上曝光 ,根据储存磷光质屏上检出的吸收度值计算12 5I rhpro UK浓度。结果与结论 该方法特异性、线性及线性范围、灵敏度、重现性、回收率均可达到蛋白多肽药物药代动力学研究的要求。并测定了 0 .8mg·kg-1静脉给药时 (12 5I rhpro UK/rhpro UK =1∶9混合 ) ,兔血浆12 5I rhpro UK浓度 -时间曲线
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| 关 键 词： | 储存磷光质屏放射自显像 药代动力学 ^125I－标记重组人尿激酶原 兔 血药浓度 蛋白多肽类 |
Assaying ~(125)I rhpro UK Concentration of Plasma in Rabbits with Storage Phosphor Screen Autoradiography |
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| YUAN Shou-jun,TANG Zhong-ming,LIU Xiu-Wen,DOU Gui-fang,SONG Hai-feng.Assaying ~(125)I rhpro UK Concentration of Plasma in Rabbits with Storage Phosphor Screen Autoradiography[J].Pharmaceutical Journal of Chinese People's Liberation Army,2001,17(1):19-21,57,58. |
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| Authors: | YUAN Shou-jun TANG Zhong-ming LIU Xiu-Wen DOU Gui-fang SONG Hai-feng |
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| Abstract: | Aim The method of assaying 125 I-rhpro-UK concentration of plasma in rabbits with combination of storage phosphor screen autoradiography and PAGE were evaluated in this paper.Methods The 125 I-rhpro-UK concentrations of plasma were calculated according to optical density on the storage phosphor screen exposed to polyacrylamide gel containing 125 I-rhpro-UK.Results and Conclusion The specificity,linear range, reproducibility and recovery for the method could meet the requirements for studying pharmacokinetics of bioactive proteins or peptides.125I-rhpro-UK concentration-time curve of plasma in rabbits was measured when giving 0.8mg*kg-1 (mixture of 125 I-rhpro-UK/I-rhpro-UK=1∶9) in vein. |
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| Keywords: | Storage phosphor screen autoradiography Pharmacokinetics 125 I lable recombinant human prourokinse Rabbit |
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