CMH为KBv_(200)耐药相关中性鞘糖脂

为了研究耐药细胞株KBv2 0 0 中性鞘糖脂表达的规律 ,采用改良的Hakomori法提取、纯化了KB及其耐药细胞株KBv2 0 0 的中性鞘糖脂 ,行高效薄层层析 ,分析其含量的异同 ;并以糖脂合成抑制剂PPMP抑制KBv2 0 0 中性鞘糖脂的合成 ,观察了其对KBv2 0 0 耐药性的逆转作用。结果发现 ,KBv2 0 0 的CMH和CDH表达较KB强 ,尤以CMH为著 ,PPMP能抑制KBv2 0 0 细胞CMH的合成 ,并能逆转KBv2 0 0 对长春新碱 (VCR)的耐药性。说明CMH为KBv2 0 0 耐药相关中性鞘糖脂 ,抑制耐药细胞CMH的合成可能是逆转肿瘤多药耐药的一种新方法。

CMH IS THE MULTIDRUG RESISTANCE(MDR) ASSOCIATED NEUTRAL GLYCOSPHINGOLIPID IN KBv_(200)CELL LINE

In order to study the relationship between the expression of neutral glycosphingolipids(N-GSLs) and MDR, N-GSLs in MDR cell line KBv 200 and its parental cell line KB were studied. The N-GSLs were extracted and purified from the KB and KBv 200 cells according to the modified method of Hakomori, then analysed by High Performance Thin Layer Chromatography (HPTLC). The effects of PPMP on the expression of N-GSLs in KBv 200 cell line were also studied by the above method, its reversion on the KBv 200 cells to Vincristine (VCR) was examined by MTT method. The results showed that the levels of CMH and CDH in KBv 200 cells were higher than in KB cells, CMH was much more markedly increased, PPMP could reverse MDR by inhibiting CMH synthesis. It suggested that CMH is MDR associated N-GSLs in KBv 200 cell line, and inhibition of CMH expression in tumor cells maybe a new way to reverse MDR.