Study on the RFLPs and amplication and rearrangement of the transforming genes in primary hepatic carcinoma,gastric carcinoma and brain tumor with six human oncogene probes |
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Authors: | Wang Shijun Shan Xiangnian Zhang Lishan Gao Yizhi Zhao Shouyuan Zhang Zhiping Li Fangyuan Zhang Qin Yan Ming Huang Ying Mao Yiping Jiang Qing He Lin |
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Institution: | 1. Department of Biology, Nanjing Railway Medical College, Nanjing, China 2. Institute of Genetics, Fudan University, Shanghai
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Abstract: | By using c-Ha-ras-1, N-ras Wigler (left sequence) and P52C-(right sequence), c-sis, v-erbB, c-myc and v-fos oncogenes as probes, restriction fragment length polymorphisms (RFLPs) of tumor tissue DNAs of 95 patients with gastric carcinoma, primary hepatic carcinoma and brain tumor, and those of 90 normal individuals were studied with the techniques of Southern blot and dot blot. Gene amplification and recombination were also examined in some tumors simultaneously. Some alleles of oncogene are reported in Chinese population for the first time. Moreover, the characteristic frequency of some “rare” alleles and genotypes occurred in some tumor samples is significantly higher than that occured in normal individuals. Pedigree analysis for 2 patients showed that some “rare” alleles are also abandant. Besides, gene amplification and recombination were found in some tumors. |
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