Video-rate high-resolution single-pixel nonscanning photoacoustic microscopy |
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| Authors: | Ningbo Chen Jia Yu Liangjian Liu Zhiqiang Xu Rongkang Gao Tao Chen Liang Song Wei Zheng Chengbo Liu |
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| Affiliation: | 1.Research Laboratory for Biomedical Optics and Molecular Imaging, CAS Key Laboratory of Health Informatics, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China;2.Shenzhen College of Advanced Technology, University of Chinese Academy of Sciences, Shenzhen 518055, China;3.National Innovation Center for Advanced Medical Devices, Shenzhen 518131, China;4.Equal contributors.; 5. ; 6. |
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| Abstract: | Optical-resolution photoacoustic microscopy (OR-PAM) is widely utilized in biomedical applications because of its ability to noninvasively image biological tissues in vivo while providing high-resolution morphological and functional information. However, one drawback of conventional OR-PAM is its imaging speed, which is restricted by the scanning technique employed. To achieve a higher imaging frame rate, we present video-rate high-resolution single-pixel nonscanning photoacoustic microscopy (SPN-PAM), which utilizes Fourier orthogonal basis structured planar illumination to overcome the above-mentioned limitations. A 473 × 473 µm2 imaging field of view (FOV) with 3.73 µm lateral resolution and video-rate imaging of 30 Hz were achieved. In addition, in both in vitro cell and in vivo mouse vascular hemodynamic imaging experiments, high-quality images were obtained at ultralow sampling rates. Thus, the proposed high-resolution SPN-PAM with video-rate imaging speed provides new insights into high-speed PA imaging and could be a powerful tool for rapid biological imaging. |
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