广玉兰果实中双和厚朴酚A提取工艺研究

目的优化广玉兰果实的有效成分双和厚朴酚A的提取工艺。方法采用反相高效液相色谱法测定不同提取溶媒中双和厚朴酚A的含量。以双和厚朴酚A得率为指标，采用L9（34）正交试验考察料液比、浸泡时间及提取次数3个因素对提取工艺的影响。结果双和厚朴酚A的最佳提取工艺条件是正己烷为提取溶媒，料液比为130、浸泡时间为48 h、提取次数为3次。结论通过正交设计法优选的双和厚朴酚A提取工艺简单、稳定可靠、重复性好。

Study on the extraction technology of bishonokiol A from Magnolia grandiflora seeds

ObjectiveTo optimize the extraction technology of bishonokiol A from the Magnolia grandiflora seeds.MethodsThe concentration of bishonokiol A in different extracting solvents was determined using RP-HPLC method.The yield rate of bishonokiol A was set as the indictor, the effects of the ratio of material-to-liquid, soaking duration, and extraction times on the extraction technology were observed using L9(34) orthogonal test.ResultsThe orthogonal test showed that the ratio of material-to-liquid for 130, soaking duration for 48 h, and extraction times for three times were the best technological condition of bishonokiol A extraction.ConclusionsThe optimized extraction process designed by orthogonal test is simple, stable and reliable.