结直肠癌AIM2和血清CEA表达水平及其临床意义

目的探讨结直肠癌患者黑色素瘤缺乏因子2（AIM2）和血清癌胚抗原（CEA）表达水平及其临床意义。 方法收集辽宁省肿瘤医院2010年1月~2013年3月118例结直肠癌患者的肿瘤组织标本及其50例癌旁正常组织标本，采用免疫组织化学法测定组织中AIM2的表达，回顾性搜集患者临床病理参数及术前通过电化学发光法（ECUA）测定的血清CEA水平。通过相关性分析癌组织中AIM2表达水平和血清CEA水平的相关性，分析两种指标与临床病理参数的关系。采用Kaplan-Meier法对不同AIM2、CEA水平组别进行生存分析。 结果118例肿瘤组织中有42例AIM2呈高表达，有39例癌旁正常组织呈高表达，差异具有统计学意义（χ²=25.295，P<0.001）；结直肠癌患者术前血清CEA阳性率为44.07%（52/118）。Spearman等级相关性分析结果显示，结直肠癌组织AIM2和血清CEA表达呈负相关（r=-0.660，P<0.001）。肿瘤的浸润深度、TNM分期以及淋巴结转移是影响癌组织AIM2表达水平的相关因素（χ²=4.847，7.794，3.961；均P<0.05）；肿瘤大小、TNM分期以及分化程度是影响患者术前血清CEA水平的相关因素（χ²=17.14，5.779，5.293；均P<0.05）。K-M生存分析显示，AIM2高表达组生存时间明显长于低表达组，术前CEA阴性组生存时间明显长于阳性组，AIM2高表达联合CEA阴性患者生存时间明显长于AIM2低表达联合CEA阳性患者，差异具有统计学意义。 结论结直肠癌患者AIM2和血清CEA表达可能与结直肠癌的进展有关，联合分析两个指标有助于评估结直肠癌患者预后。

Expression of AIM2 and serum CEA level in patients with colorectal cancer and its clinical significance

ObjectiveTo investigate the expression of absent in melanoma 2 (AIM2) and serum carcinoembryonic antigen (CEA) in patients with colorectal cancer and its clinical significance. Methods118 cancer tissues of colorectal cancer and 50 normal tissues were collected from Liaoning Cancer Hospital from January 2010 to March 2013. The expression of AIM2 in colorectal cancer tissues was detected by immunohistochemistry. Clinical and pathological parameters and serum CEA levels were retrospectively collected. The correlation between AIM2 expression level and serum CEA level in cancer tissues was analyzed by correlation analysis, and the relationship between the two indicators and clinicopathological parameters was analyzed. Kaplan-Meier method was used to analyze the survival of different AIM2 and CEA levels. ResultsThere were 42 cases of AIM2 overexpression in 118 and 39 cases in normal tissues. The difference was statistically significant; Preoperative serum CEA positive rate in patients with colorectal cancer was 44.07%(52/118). Spearman grade correlation analysis showed that AIM2 expression in colorectal cancer tissues was negatively correlated with CEA expression in serum (r=-0.660, P<0.001). The depth of invasion, TNM stage and lymph node metastasis were related factors affecting the expression of AIM2 in cancer tissues (χ²=4.847, 7.794, 3.961; P<0.05); the size of tumors, TNM stage and differentiation were related factors affecting the level of serum CEA before operation (χ²=17.14, 5.779, 5.293; P<0.05). K-M survival analysis showed that the survival time of AIM2 high expression group was significantly longer than that of low expression group, the preperative survival time of CEA negative group was significantly longer than that of positive group, and the survival time of AIM2 high expression+ CEA negative group was significantly longer than that of AIM2 low expression+ CEA positive group, the difference was statistically significant. ConclusionThe expression of AIM2 and serum CEA in patients with colorectal cancer may be related to the progression of colorectal cancer. Combined analysis of the two indicators is helpful to evaluate the prognosis of patients with colorectal cancer.