血必净对水下爆炸致兔急性闭合性肝损伤早期干预的效果研究

目的探讨血必净对水下爆炸致兔急性闭合性肝损伤早期干预的效果研究。方法健康新西兰兔15只，随机分为对照组、损伤组和用药组（给予血必净），以水下爆炸致兔急性闭合性肝损伤，用酶联免疫法检测爆炸后0、4、12、24 h血清中丙氨酸氨基转移酶（ALT）、白细胞介素（IL）-6、基质金属蛋白酶（MMP）-9的浓度。结果爆炸后4 h各组间ALT差异有统计学意义（P < 0.05），损伤组水平高于对照组（P < 0.05）；爆炸后12 h各组间IL-6水平差异有统计学意义（P < 0.01），损伤组水平高于对照组和用药组（P < 0.05~P < 0.01）；爆炸后24 h MMP-9水平各组间差异有统计学意义（P < 0.05），损伤组水平高于对照组和用药组（P < 0.05）；损伤组爆炸后4 h与爆炸后12 h和24 h MMP-9间水平差异有统计学意义（P < 0.05），用药组爆炸后4 h与爆炸后12 h MMP-9间水平差异有统计学意义（P < 0.05）。结论血必净对水下爆炸致兔急性闭合性肝损伤有保护作用。

Study on the effect of Xuebijing on the early intervention of acute closed liver injury induced by underwater explosion

ObjectiveTo explore the effects of Xuebijing on the early intervention of acute closed liver injury induced by underwater explosion.MethodsFifteen healthy New Zealand rabbits were randomly divided into the control group, injury group and intervention group(treated with Xuebijing), and the acute closed hepatic injury in three groups was established by underwater explosion.The serum levels of ALT, IL-6 and MMP-9 in three groups after 0, 4, 12 and 24 h of explosion were detected using enzyme-linked immunoassay.ResultsThe differences of the ALT levels among three groups after 4 h of explosion were statistically significant(P < 0.05), and the level of ALT in injury group was higher than that in control group(P < 0.05).The differences of the IL-6 levels among three groups after 12 h of explosion were statistically significant(P < 0.01), and the level of IL-6 in injury group was higher than that in control group and intervention group(P < 0.05 to P < 0.01).The differences of the MMP-9 levels among three groups after 24 h of explosion were statistically significant(P < 0.05), and the level of MMP-9 in injury group was higher than that in control group and intervention group(P < 0.05).The differences of the MMP-9 levels in injury group between after 4 h and 12 h, 24 h of explosion were statistically significant(P < 0.05), and the difference of the MMP-9 level in intervention group between after 4 h and 12 h of explosion was statistically significant(P < 0.05).ConclusionsXuebijing plays a protective effect on the acute closed liver injury caused by underwater explosion in rabbits.