马齿苋提取物治疗大鼠口腔溃疡的疗效及其对细胞免疫、 炎性因子的影响

目的观察马齿苋提取物治疗大鼠口腔溃疡的疗效，初步探讨其对大鼠细胞免疫、炎性因子的影响，以期为临床用药提供参考。方法采用抗原乳化液方法建立大鼠口腔溃疡模型，实验设有空白对照组、模型对照组（不给予药物处理）、口腔溃疡散组（6%口腔溃疡散混悬液涂抹）以及马齿苋提取物组（0.24 g/mL马齿苋水溶液灌胃），每组8只大鼠。观察各组大鼠口腔溃疡数目、持续时间以及治疗效果。比较各组大鼠的血清干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、白细胞介素-2（IL-2）水平以及T细胞亚群水平变化情况。结果各组大鼠口腔溃疡数目、持续时间差异均有统计学意义（P < 0.01），且马齿苋提取物组显著低于口腔溃疡散组和模型对照组（P < 0.05~P < 0.01），口腔溃疡散组显著低于模型对照组（P < 0.01和P < 0.05）。口腔溃疡散组小鼠治疗效果优于模型对照组，马齿苋提取物组小鼠治疗效果优于口腔溃疡散组与模型对照组（P < 0.05）。各组大鼠炎性因子水平差异均有统计学意义（P < 0.01），且血清IFN-γ水平，模型对照组显著低于空白对照组，口腔溃疡散组与马齿苋提取物组明显高于模型对照组，马齿苋提取物组高于口腔溃疡散组（P < 0.05~P < 0.01）；血清TNF-α和IL-2水平，模型对照组较空白对照组明显升高，口腔溃疡散组、马齿苋提取物组低于模型对照组（P < 0.01），马齿苋提取物组与口腔溃疡散组间差异无统计学意义（P>0.05）。各组大鼠T淋巴细胞亚群水平差异均有统计学意义（P < 0.01），且模型对照组CD3+、CD4+和CD4+/CD8+水平显著低于空白对照组，CD8+水平显著高于空白对照组（P < 0.01）；口腔溃疡散组与马齿苋提取液组CD3+、CD4+和CD4+/CD8+水平均高于模型组，而CD8+均低于模型对照组（P < 0.05~P < 0.01），马齿苋提取液组CD3+、CD4+和CD4+/CD8+水平均明显高于口腔溃疡散组（P < 0.05~P < 0.01）。结论马齿苋提取物可有效缩短大鼠溃疡持续时间，减少溃疡数目，提高溃疡治疗效率。其机制可能是通过增强机体免疫功能以治疗复合性口腔溃疡。

Efficacy of purslane extract in the treatment of oral ulcer,and its effects on cellular immunity and inflammatory factors in rats

ObjectiveTo observe the therapeutic effects of purslane extract on oral ulcer, and explore its effects on cellular immunity and inflammatory factors in rats, to provide reference for clinical drug use.MethodsThe rat oral ulcer model was established by antigen emulsion method.The rats were divided into the blank control group, model control group (no medication), oral ulcer powder group (treatment with oral ulcer powder suspension painting) and purslane extract group (treatment with purslane solution gavage) (8 rats each group).The number, duration and therapeutic effect of oral ulcer in all groups were observed.The levels of serum interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) and T cell subsets were compared among all groups.ResultsThe differences of the number and duration of oral ulcer among all groups were statistically significant (P < 0.01), which in purslane extract group were significantly lower than those in oral ulcer powder group and model control group (P < 0.05 to P < 0.01), and which in oral ulcer powder group was significantly lower than that in model control group (P < 0.01 and P < 0.05).The therapeutic effect in oral ulcer powder group was better than that in model control group, and which in purslane extract group was better than that in oral ulcer powder group and model control group (P < 0.05).The differences of the serum levels of inflammatory factors among all groups were statistically significant (P < 0.01), the serum level of IFN-γ in model control group was significantly lower than that in blank control group, the serum levels of IFN-γ in oral ulcer powder group and purslane extract group were significantly higher than that in model control group, and the serum level of IFN-γ in purslane extract group was higher than that in oral ulcer powder group (P < 0.05 to P < 0.01).The serum levels of TNF-α and IL-2 in model control group significantly increased compared with the blank control group, which in oral ulcer powder group and purslane extract group were lower than those in model control group (P < 0.01), and the differences of the serum levels of TNF-α and IL-2 between oral ulcer powder group and purslane extract group were not statistically significant (P>0.05).The differences of the T lymphocyte subsets levels among all groups were statistically significant (P < 0.01), the levels of CD3+、CD4+ and CD4+/CD8+ in model control group were significantly lower than those in blank control group, and the serum level of CD8+ in model control group was significantly higher than that in blank control group (P < 0.01).The serum levels of CD3+, CD4+ and CD4+/CD8+ in oral ulcer powder group and purslane extract group were higher than those in model control group (P < 0.01), the serum levels of CD8+ in oral ulcer powder group and purslane extract group were lower than that in model control group (P < 0.01), and the serum levels of CD3+, CD4+ and CD4+/CD8+ in purslane extract group were significantly higher than those in oral ulcer powder group (P < 0.05 to P < 0.01).ConclusionsThe purslane extract can effectively shorten the duration of ulcer, reduce the number of ulcers, and improve the efficiency of ulcer treatment.Its mechanism may be retated to enhance the immune function of body.