雪菊中一种二氢黄酮醇糖苷对小鼠酒精性急性胰腺炎的作用研究

  目的  探讨雪菊中黄酮类化合物(2R, 3R)-二氢槲皮素7-O-β-D-吡喃葡萄糖(C1)可否减轻脂肪酸乙酯诱导的小鼠酒精性急性胰腺炎(FAEE-AP)的损伤。  方法  将30只健康SPF级小鼠随机分为对照组、模型组、低剂量组、中剂量组及高剂量组，每组6只。除对照组外，其他组小鼠均采用2次腹腔注射1.75 g/kg乙醇和200 mg/kg棕榈油酸的混合物，诱导酒精性急性胰腺炎模型。低、中、高3个剂量组在第0 h、4 h和8 h分别予以12.5、25、50 mg/kg C1腹腔注射。造模后24 h，检测其血清淀粉酶、脂肪酶和白细胞介素(IL)-6水平，胰腺组织胰蛋白酶活性，胰腺和肺组织髓过氧化物酶(MPO)活性，HE染色观察胰腺组织病理学改变，并进行免疫组织化学染色检测胰腺组织中核因子-E2相关受体2(Nrf2)的表达。  结果  模型组胰腺组织病理评分，血清淀粉酶、脂肪酶和胰腺胰蛋白酶活性，血清IL-6水平、胰腺和肺的MPO活性均高于对照组(P < 0.01)。与模型组相比，低剂量(12.5 mg/kg)组的胰腺组织病理明显改善，评分差异有统计学意义(P < 0.05)，且血清淀粉酶、脂肪酶和胰腺胰蛋白酶活性，血清IL-6水平、胰腺和肺的MPO活性降低(P < 0.05)，并能上调胰腺组织中Nrf2表达。  结论  12.5 mg/kg C1能够通过增强Nrf2的表达，下调炎性因子IL-6，减轻FAEE-AP的损伤。

Protective Effect of a Dihydroflavonol Glycoside from Coreopsis tinctoria Nutt. in Mouse Model of Alcoholic Acute Pancreatitis

  Objective  To investigate the protective effect of (2R, 3R)-dihydroquercetin 7-O-β-D-glucopyranose (C1) extracted from Coreopsis tinctoria Nutt. in a mouse model of alcoholic acute pancreatitis (FAEE-AP) induced byfatty acid ethyl ester (FAEE).  Methods  The 30 healthy SPF mice were randomly divided into control group, model group, low dose group, middle dose group and high dose group, 6 in each group. Alcoholic pancreatitis was induced by ethanol and palmitoleic acid administration (1.75 g/kg ethanol, 200 mg/kg palmitoleic acid, 2 times peritoneal injections). The three treatment groups were given C1 (0 h, 4 h, 8 h) at the dose of 12.5, 25 and 50 mg/kg, respectively. After 24 h of molding, the serum amylase, lipase and IL-6 levels were detected. The trypsin level in pancreatic tissue and myeloperoxidase (MPO) level in pancreatic and lung tissue were detected. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of pancreatic tissue and immunohistochemical (IHC) staining was used to detect the expression of nuclear factor-erythroid 2 related factor 2 (Nrf2) in pancreatic tissue.  Results  The pancreatic histopathological scores, serum amylase and lipase activity, trypsin level in pancreatic tissue, serum IL-6 level, MPO level of pancreas and lung were significantly higher in the model group than in the control group (P < 0.01). Compared with the model group, the pancreatic histopathologies of the low dose group was significantly improved (P < 0.05), as well as the serum amylase and lipase activity, trypsin level of pancreas, serum IL-6 level, the pancreas andthe lung's MPO level decreased significantly (P < 0.05), and up-regulate that expression of Nrf2 in pancreatic tissue.  Conclusion  12.5 mg/kg of (2R, 3R) -dihydroquercetin 7-O-β-D-glucopyranose (C1) improved the expression of Nrf2, reduced the expression of inflammatory factor IL-6, and protected acute pancreatitis caused by FAEE.