淫羊藿对兔腰椎间盘退变模型软骨组织中BMP-2、BMP-4表达水平的影响

目的探讨淫羊藿对兔腰椎间盘退变模型软骨组织骨形态发生蛋白（BMP）-2、BMP-4表达的影响。方法取30只新西兰大白兔，分为假手术组、模型组、淫羊藿组各10只，除假手术组外均建立腰椎间盘退变模型，淫羊藿组给予10 mg·kg-1·d-1淫羊藿药液灌胃，模型组给予同等剂量0.9%氯化钠溶液灌胃，90 d收集各组腰椎软骨终板标本，观察组织形态改变情况，采用免疫组织化学法测定凋亡因子fas受体表达，采用Western blotting检测软骨终板组织中BMP-2、BMP-4蛋白表达情况。结果模型组、淫羊藿组fas受体阳性率均高于假手术组（P < 0.01），淫羊藿组fas受体阳性率低于模型组（P < 0.01）；模型组、淫羊藿组BMP-2蛋白表达水平低于假手术组（P < 0.01），淫羊藿组BMP-2蛋白表达水平高于模型组（P < 0.01），各组BMP-4蛋白表达水平差异均无统计学意义（P>0.05）。结论淫羊藿可上调兔腰椎间盘退变模型软骨组织BMP-2表达，促进成骨细胞增殖、分化，但对BMP-4的调控仍无实验依据。

Effect of epimedium on the expression levels of BMP-2 and BMP-4 in cartilage tissue of rabbit model with lumbar intervertebral disc degeneration

ObjectiveTo investigate the effects of epimedium on the expression levels of bone morphogenetic protein -2(BMP-2) and -4(BMP-4) in cartilage tissue of rabbit model with lumbar intervertebral disc degeneration.MethodsThirty New Zealand white rabbits were divided into the sham operation group, model group and epimedium group (10 rabbits each group), and the lumbar intervertebral disc degeneration rabbit models in other two groups were established except for the sham operation group.The epimedium group and model group were treated with intragastric administration of 10 mg·kg-1·d-1 epimedium solution and same volume of 0.9% sodium chloride injection, respectively.The lumbar cartilage endplate specimens were harvested from each group after 90 d of treatment, and the morphological change of tissue was observed.The expression levels of apoptosis factor fas receptor, and BMP-2 and BMP-4 protein in cartilage endplate were determined using immunohistochemistry and Western blotting, respectively.ResultsThe positive rates of fas receptor in model group and epimedium group were higher than that in sham operation group (P < 0.01), and the positive rate of fas receptor in epimedium group was lower than that in model group (P < 0.01).The expression levels of BMP-2 and BMP-4 protein in model group and epimedium group were lower than those in sham operation group (P < 0.01), the expression level of BMP-2 protein in epimedium group was higher than that in model group (P < 0.01), and there was no statistical significance in the expression level of BMP-4 among three groups (P>0.05).ConclusionsEpimedium can up-regulate the expression level of BMP-2 in cartilage tissue of rabbit model with lumbar intervertebral disc degeneration, promote the proliferation and differentiation of osteoblast, but there is no experimental basis for regulating BMP-4.