| 应用两种麦管系统对小鼠囊胚的玻璃化冷冻 |
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| 引用本文： | 朱亮,罗琛,黄敏珍,李红,邢福祺.应用两种麦管系统对小鼠囊胚的玻璃化冷冻[J].南方医科大学学报,2003,23(9):992-995. |
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| 作者姓名： | 朱亮 罗琛 黄敏珍 李红 邢福祺 |
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| 作者单位： | 1. 广州第一军医大学,南方医院生殖医学中心,广东,广州,510515
2. 广州第一军医大学,遗传学教研室,广东,广州,510515 |
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| 摘 要： | 目的比较两种麦管系统对小鼠囊胚的玻璃化冷冻比较,以期建立一种有效的囊胚冷冻方法。方法分别应用开放麦管系统和传统麦管系统对小鼠扩张囊胚进行玻璃化冷冻,比较冷冻复苏以后囊胚的存活率和孵出率。结果应用开放麦管系统冷冻的小鼠囊胚复苏后的存活率(82.3%)显著高于应用传统麦管系统者(68.6%, P<0.01)。前者冷冻的小鼠囊胚复苏后的囊胚孵出率(70.3%),也明显高于应用传统麦管系统者(57.1%, P<0.05)。结论应用开放性麦管系统进行小鼠囊胚的玻璃化冷冻是一种行之有效的冷冻方法。
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| 关 键 词： | 开放性麦管系统 小鼠囊胚 玻璃化冷冻 辅助生殖 |
| 文章编号： | 1000-2588(2003)09-0992-04 |
| 修稿时间： | 2003年1月13日 |
Vitrification of mouse blastocysts using two kinds of straw systems |
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| ZHU Liang ,LOU Chen ,HUANG Min-zhen ,LI Hong ,XING Fu-qi Center of Assisted Reproduction,Nanfang Hospital.Vitrification of mouse blastocysts using two kinds of straw systems[J].Journal of Southern Medical University,2003,23(9):992-995. |
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| Authors: | ZHU Liang LOU Chen HUANG Min-zhen LI Hong XING Fu-qi Center of Assisted Reproduction Nanfang Hospital |
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| Institution: | ZHU Liang 1,LOU Chen 2,HUANG Min-zhen 1,LI Hong 1,XING Fu-qi 11 Center of Assisted Reproduction,Nanfang Hospital,2 Department of Genetics,First Military Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To compare open pulled straws with conventional sorans system for the purpose of establishing an effective vitrification method for mouse blastocysts. Methods With open pulled straws (OPS) and conventional straws, vitrifi- cation of mouse blastocysts was performed, and the survival and hatching rates of the blastocysts were observed after thawing. Results After vitrification, the embryo survival in the OPS group (82.3%) was significantly higher than conventional straw group (68.6%, P<0.01), and the hatching rate was also higher (70.3% vs57.1%, P<0.05). Conclusion Vitrification of mouse blastocysts using OPS results in higher survival and hatching rates as compared with conventional straw method. |
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| Keywords: | open pulled straws(OPS) mouse blastocysts vitrification assisted reproductive technique |
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