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Plasmodium vivax circumsporozoite variants and Duffy blood group genotypes in the Brazilian Amazon region |
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| Authors: | Storti-Melo Luciane M de Souza-Neiras Wanessa C Cassiano Gustavo C Joazeiro Ana C P Fontes Cor J Bonini-Domingos Cláudia R Couto Alvaro A R D'Almeida Povoa Marinete M de Mattos Luiz C Cavasini Carlos E Rossit Andréa R B Machado Ricardo L D |
| Institution: | a University of São Paulo State Júlio Mesquita Filho, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, São Paulo State, Brazil b Faculty of Medicine of São José do Rio Preto, Avenida Brigadeiro Faria Lima 5416, Vila São Pedro, São José do Rio Preto, São Paulo State, Brazil c Mato Grosso Federal University, Department of Medical Clinic, Rua Luiz Phellipe Pereira Leite, 78048-902 Cuiabá, Mato Grosso State, Brazil d SEAMA Faculty, Macapá, Amapá State, Brazil e Evandro Chagas Institute, MS/SVS, Rodovia BR-316 km 7 s/n, Levilândia 67030-000, Ananindeua, Pará State, Brazil f Faculty of Medicine of São José do Rio Preto Foundation, Avenida Brigadeiro Faria Lima 5416, 15090-000 São José do Rio Preto, São Paulo State, Brazil |
| Abstract: | The circumsporozoite protein (CSP) of the Plasmodium vivax infective sporozoite is considered to be a major target for the development of recombinant malaria vaccines. The Duffy blood group molecule acts as the red blood cell receptor for P. vivax. We review the frequency of P. vivax CSP variants and report their association with the Duffy blood group genotypes from Brazilian Amazon patients carrying P. vivax malaria. Peripheral blood samples were collected from 155 P. vivax-infected individuals from five Brazilian malaria-endemic areas. The P. vivax CSP variants and the Duffy blood group genotypes were assessed using PCR/RFLP. In single infections, the VK210 variant was the commonest followed by the P. vivax-like variant. The typing of P. vivax indicated that the frequency of variants among the study areas was significantly different from one to another. This is the first detection of the VK247 and P. vivax-like variant in single infections in endemic areas of Brazil. Association of the CSP P. vivax variants with the heterozygous Duffy blood group system genotype was significant for VK210 single infection. These observations provide additional data on the Plasmodium-host interactions concerning the Duffy blood group and P. vivax capability of causing human malaria. |
| Keywords: | Malaria Plasmodium vivax Circumsporozoite protein Genotype Duffy blood group system Brazil |
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