|
|||||
|
|
Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations |
|
| Authors: | Nkouawa Agathe Okamoto Munehiro Mabou Alida Kouojip Edinga Eulodie Yamasaki Hiroshi Sako Yasuhito Nakao Minoru Nakaya Kazuhiro Blair David Agatsuma Takeshi Enyong Peter Shibahara Toshiyuki Moyou-Somo Roger Ito Akira |
| Affiliation: | a Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan b Medical Research Center, Institute of Medical Research and Study of Medicinal Plants (IMPM), Yaounde, Cameroon c Department of Laboratory Animal Science, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan d District Hospital of Palm City, Ministry of Health, Douala, Cameroon e Department of Parasitology and Infectious Diseases, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon f Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan g School of Marine and Tropical Biology, James Cook University, Townsville, Queensland, Australia h Department of Environmental Health Science, Faculty of Medicine, Kochi University, Kochi, Japan i Division of Laboratory Animal Science, Research Center for Bioscience and Technology, Tottori University, Tottori, Japan |
| Abstract: | Paragonimiasis is a common parasitic zoonosis in Cameroon and neighbouring countries in Western Africa. Serum, sputum and faecal samples were collected in an endemic area of South West Province, Cameroon, after administration of a questionnaire to identify individuals with appropriate symptoms and histories. Microscopic examination revealed eggs in sputum from 16 people, but none in any faecal sample. These 16 were among the 25 and 26 people, respectively, positive by ELISA and by immunoblot using Paragonimus africanus crude antigens. Copro-DNA detection was attempted using 23 faecal samples (18 from sputum egg-negative and five from sputum egg-positive individuals). Copro-DNA was detected in four of the five sputum egg-positive individuals. These results strongly suggest that: (1) serology is much more sensitive than sputum examination for diagnosis of paragonimiasis; and (2) a copro-DNA test may be more sensitive than a microscopic search for eggs in faeces. Molecular sequence data from ITS2 and cox1 genes confirmed that adult worms experimentally raised in cats were P. africanus and that eggs from sputum or other worm products from human faeces also belonged to this species. Based on these results, 26 of 168 persons (15.5%) were diagnosed as suffering from paragonimiasis. |
| Keywords: | Paragonimiasis Paragonimus africanus Diagnosis Serology Copro-DNA Cameroon |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |