活化的T细胞促进间充质干细胞分化为成骨细胞

本研究旨在探讨活化的T细胞对间充质干细胞（mesenchymal stem cell，MSC）向成骨细胞分化的影响。将活化的T细胞与MSC共培养，14天后碱性磷酸酶染色，观察成骨细胞的生成。此外，在MSC的培养体系中加入活化的T细胞上清，RT—PCR检测活化的T细胞免疫相关分子的表达，探讨哪些细胞因子参与了MSC向成骨细胞分化。结果表明，活化的T细胞能促进MSC分化为成骨细胞，IL-1β在其中发挥重要的作用，而TNF-α，TGF—β1作用不明显一、结论：活化的T细胞促进MSC向成骨细胞分化，MSC与免疫细胞通过细胞因子发生相互作用。

Activated T Cells Promote Differentiation of Mesenchymal Stem Cells into Osteoblasts

This study was aimed to investigate the effect of activated T cell on the ability of MSC to differentiate into osteoblasts. The activated T cells with MSCs were co-culture for 14 days, then the osteoblast formation was tested by alkaline phosphatase staining. Furthermore, the supernatant of activated T cell was added in culture system of MSCs, the expression of molecules related with immune regulation of activated T cells was detected by RT-PCR, so as to determine what kinds of cytokine displayed the important function in MSC differentiation. The result showed that activated T cell could promote differentiation of MSC into osteoblasts, and IL-1β played an important role in the effect of activated T cells on MSCs, while TNF-α, TGF-1β were not. It is concluded that the activated T cells promote the differentiation of MSCs to osteoblasts. The interatcive influence between MSCs and immune cells can be mediated through cytokines.