Novel PCR-restriction fragment length polymorphism analysis for rapid typing of staphylococcal cassette chromosome mec elements |
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| Authors: | Yang Jin Ah Park Dae Won Sohn Jang Wook Kim Min Ja |
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| Affiliation: | Graduate School of Life Science and Biotechnology, College of Medicine, Korea University, Seoul. |
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| Abstract: | We developed a novel PCR-restriction fragment length polymorphism test for the ccrB gene by using HinfI and BsmI for rapid typing of staphylococcal cassette chromosome mec (SCCmec). When tested with reference strains and methicillin-resistant Staphylococcus aureus isolates, the method proved to be valid and useful for rapid identification of four SCCmec types, especially type IV. |
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