波浪床结合封闭式负压引流对压力性溃疡创面渗液活性的影响

目的 观察创面渗液对体外成纤维细胞生长的影响,探讨促进压力性溃疡愈合的方法及其机制.方法 共选取18例压力性溃疡患者,将其随机分成治疗组(波浪床结合封闭式负压引流,n=8)和对照组(波浪床结合传统换药方法,n=10),收集第1,3,7天创面渗液,进行体外成纤维细胞生长干预实验.结果 第1天两组渗液都抑制细胞的生长；对照组第3天渗液仍然抑制细胞生长,而实验组却促进细胞生长；两组第7天的渗液都促进细胞的生长.结论 慢性创面渗液抑制成纤维细胞生长,波浪床结合封闭式负压引流治疗压力性溃疡能更快的改变创面渗液的活性以促进成纤维细胞增生.

Effect of the Pressure Ulcer Wound Fluid to Fibroblast Proliferation by Multi-wave Bed Combined together with Vacuumassisted Closure

Objective To observe the effect of wound fluids in growth of flbroblast in vitro and further investigates the method and mechanism to accelerate the healing of pressure ulcer. Methods 18 patients with pressure ulcer were involved and randomly allocated to treatment group （waves-bed and vacuum-assisted closure, n =8） and control group （waves-bed and ordinary dressing, n =10）. Wound fluids were collected in the first, third, seventh day and used to interfere in the growth of fibroblast in vitro. Results Both of wound fluids from two groups inhibited the growth of fibroblast in the first day; wound fluids from control group remained to inhibit cell growth while treatment group promoted in the third day; both of wound fluids accelerated cell growth in the seventh day. Conclusion Fluids in chronic wound inhibit the growth of fibroblast. The treatment of pressure ulcer with waves-bed and vacuum-assisted closure can elevate the activity of wound fluids to accelerate the proliferation of fibroblast.