贲门腺癌组织中Smad基因家族不同成员的表达及其临床意义

目的:检测贲门腺癌(gastric cardia adenocarcinoma,GCA)中Smad基因家族不同成员的表达水平及其相关性,并探讨其临床意义。方法:收集河北医科大学第四医院2004至2009年间病理确证的贲门腺癌患者110例,RT-PCR检测贲门腺癌和癌旁组织中Smad2、Smad3、Smad4和Smad7 mRNA的表达,免疫组织化学方法检测贲门腺癌组织和癌旁组织中p-Smad2/3、Smad4和Smad7蛋白的表达并分析其相关性,分析p-Smad2/3、Smad4和Smad7蛋白表达与贲门腺癌临床病理特征的关系。结果:贲门腺癌组织中Smad2、Smad3和Smad4 mRNA表达水平均显著低于相应癌旁组织(0.4956±0.1862)vs(0.8611±0.2914),P<0.01;(0.4713±0.1712)vs(0.8314±0.2811),P<0.01;(0.5145±0.1987)vs(0.8954±0.2856),P<0.01],而Smad7 mRNA表达水平显著高于癌旁组织(0.5114±0.1962)vs(0.2012±0.1006),P<0.01]。贲门腺癌组织p-Smad2/3、Smad4蛋白表达的阳性率显著低于癌旁组织(42.7%vs 93.6%,P<0.01;45.5%vs 95.5%,P<0.01),且与肿瘤TNM分期和组织分化程度密切相关(P<0.05)。贲门腺癌组织Smad7蛋白表达阳性率显著高于癌旁组织(48.2%vs 3.6%,P<0.01),且与肿瘤组织分化程度密切相关(P<0.05)。贲门腺癌中p-Smad2/3与Smad4蛋白的表达呈正相关,但它们均与Smad7蛋白表达无明显的相关性。结论:贲门腺癌组织低表达Smad2、Smad3和Smad4,高表达Smad7,Smad基因的异常表达可能参与贲门腺癌的发生、发展。

Expressions of different Smads gene family members in gastric cardia adenocarcinoma tissues and their clinical significance

Objective:To investigate the expressions of different Smad genes in gastric cardia adenocarcinoma(GCA) and to explore their correlation and clinical significance.Methods: 110 cases of pathologically confirmed GCA were included from the Fourth Hospital of Hebei Medical University during 2004-2009 year.RT-PCR and immunohistochemistry methods was used to respectively detect the mRNA and protein expressions of Smad2,Smad3,Smad4 and Smad7 in GCA.Their correlations with clinical pathological characteristics of GCA were analyzed.Results: The expressions of Smad2,Smad3 and Smad4 mRNA in GCA tissues were significantly reduced with comparison to the paired normal tissues(0.4956±0.1862] vs 0.8611±0.2914],P<0.01;0.4713±0.1712] vs 0.8314±0.2811],P<0.01;0.5145±0.1987] vs 0.8954±0.2856],P<0.01),and Smad7 mRNA expression in GCA tissues was significantly increased with comparison to the paired normal tissues(0.5114±0.1962] vs 0.2012±0.1006],P<0.01).The positive protein expression rate of p-Smad2/3 and Smad4 in GCA tissues was significantly lower than that in the paired normal tissues(42.7% vs 93.6%,P<0.01;45.5% vs 95.5%,P<0.01).The protein expressions of p-Smad2/3 and Smad4 were associated with TNM stage and pathological differentiation(P<0.05).The positive protein expression rate of Smad7 in GCA tissues(48.2%) was significantly higher than that in the paired normal tissues(3.6%) and was associated with pathological differentiation(P<0.05).The protein expression of p-Smad2/3 and Smad4 was positively correlated,while p-Smad2/3 and Smad4 did not show any correlation with Smad7.Conclusion: Decreased expression of Smad2,Smad3 and Smad4 and increased expression of Smad7 in GCA may be associated with the occurrence and development of GCA.