抗原刺激后小鼠CD4~＋ T细胞IL-10受体Ⅰ表达情况的研究

目的：检测抗原刺激后小鼠CD4＋T淋巴细胞表面白细胞介素10受体Ⅰ（interleutin-10 receptorⅠ,IL-10R1）表达变化情况。方法：取C57BL/6小鼠脾细胞,溶血后采用尼龙毛去除B淋巴细胞后,流式细胞仪无菌分选CD4＋T淋巴细胞,利用anti-CD3和anti-CD28多克隆抗体刺激分选后细胞,分别在第0、12、24、48和72 h应用流式细胞术检测IL-10R1表达情况。结果：0 h时小鼠CD4＋T淋巴细胞不表达IL-10R1,随着刺激时间的延长IL-10R1表达增加,24 h时达到最高,随后表达水平开始下降,72 h时表达情况与0 h基本相同。结论：白细胞介素10（interleutin-10,IL-10）对小鼠CD4＋T淋巴细胞的调节可能是在IL-10R1短暂表达的72 h内。

The Interleutin-10 Receptor I Expression on the Mouse CD4 + T Cell after the Stimulation by Antigen

Objective:To study the interleutin-10 receptor Ⅰ(IL-10R1) expression on the mouse CD4+ T cell after the stimulation by antigen.Methods:Spleen cells of C57BL/6 mouse were isolated and B cells were got rid of by nylon filter after hemolysis.CD4+T cells were isolated sterilely by flow cytometry.The IL-10R1 level on the CD4+T cell at the 0 h,12 h,24 h,48 h and 72 h after stimulation were detected by anti-CD3 and anti-CD28 polyclonal antibody.Results:At the 0th hour,CD4+T cell did not express IL-10R1.With the extension of time the level of IL-10R1 were increased and at the 24 th hour reached the peak.Then the level of IL-10R1 began to fall and at 72 th hour the level of IL-10R1 was similar with the level at 0th hour.Conclusion:IL-10 regulating CD4+T cell may be in the 72 hours of IL-10R1 expression.