猪离体不停跳心脏模型建立和应用

目的 研究不停跳技术在猪离体心脏实验模型的应用.方法 7只实验用猪,戊巴比妥钠麻醉后常规开胸,在不停跳的情况下游离血管,建立体外循环管道连接,利用体外循环机保证冠脉灌注.在心脏不停跳情况下进行心脏离体手术,离体后心脏置无菌塑料袋于恒温水箱(37℃)保存,左心耳置引流管.灌注血液温度37℃,流量控制在300 ml/min左右.观察离体后心脏跳动情况,离体后每隔1小时取左心耳组织测定丙二醛(MDA)、超氧化歧酶(SOD),6小时后取心尖部心肌行电镜检测.结果 7只动物除一只因左心反流胀停失败,其余均成功建立离体不停跳模型.MDA及SOD在离体6h内变化不明显,心肌超微结构保持良好.结论 在体外循环系统支持下可以保证心脏在离体及体外过程中不停跳,避免了缺血再灌注损伤,为大型动物的体外心脏实验提供了良好的模型.

Establishment and Application of Isolated Beating Pig Heart Model

Objective To study the establishment of isolated beating heart experimental model. Methods 7 pigs were anesthetized with sodium pentobarbital then received thomcotomy, using cardiopulmonary bypass machine to ensure coronary perfusion. Separated hearts were preserved in sterile plastic bags at constant-temperature water-bath （37℃）, the left atrial appendage setting tube. Perfusion blood temperature was 37℃, flow control was 300 ml/min. Observe states of the beating heart, at intervals each of 1 h, the leg atrial appendage was resected, measuring MDA, SOD. After 6 h, apex myocardium was taken for electron microscopy. Results 1 case failed because of left ventrical reverse flow, and the others were successfully established beating heart model in vitro. MDA and SOD didn＇t change significantly within 6 h, myocardium ultrastructure maintained in good state. Conclusion The CPB system can Support heart beating in procuring surgery and in vitro, avoiding the ischemic-reperfusion injury, which provides an optimal model for cardiac experiment of large animals in vitro.