青蒿琥酯对迟发型超敏反应小鼠脾脏T淋巴细胞的免疫调节作用

背景:青蒿琥酯是青蒿素低毒、高效的衍生物,具有免疫调节功能,但其具体机制仍需研究阐明。目的:初步探讨青蒿琥酯对迟发型超敏反应小鼠脾脏T淋巴细胞的免疫调节作用。方法:建立迟发型超敏反应小鼠模型,40只小鼠随机数字表法均分正常对照组、青蒿琥酯干预组、p-38 MAPK抑制剂组、基质对照组进行实验观察。T淋巴细胞转化实验检测小鼠T淋巴细胞增殖水平;Western blot方法检测p38丝裂酶原激活蛋白激酶(p38 MAPK)蛋白的活性表达。结果与结论:局部给药后青蒿琥酯明显减轻迟发型超敏反应小鼠耳肿胀、降低脾指数、抑制刀豆蛋白A诱导的T淋巴细胞增殖;同时减弱p38MAPK的磷酸化活性表达。提示青蒿琥酯可以有效抑制小鼠迟发型超敏反应,其作用途径可能与抑制p38MAPK信号通路有关。

Immunomodulatory activity of artesunate to T lymphocytes from the spleens of delayed-type hypersensitivity mice

BACKGROUND:Artesunate is a derivant of high efficiency and low toxicity from artemisinin,which has been shown to affect immune response.However,the underlying mechanism remains elusive.OBJECTIVE:To investigate the immunoregulatory mechanism of artesunate to T lymphocytes from the spleens of delayed-type hypersensitivity mice.METHODS:The effects of artesunate on the ear swelling and spleen index were measured in delayed-type hypersensitivity mice.The transformation of spleen lymphocytes was used to detect the proliferation of T cells.Western blot assay was applied to investigate the p38 mitogen-activated protein kinase(MAPK) activation.The proliferation of T lymphocytes was detected by T lymphocyte transformation test.MAPK activity was detected by western blot method.RESULTS AND CONCLUSION:Topical application of artesunate could suppress the increase in ear thickness,spleen index and ConA-induced T lymphocytes proliferation significantly.Furthermore,artesunate was observed to decrease the phosphorylation of p38 MAPK.These demonstrated that artesunate had an anti-delayed-type hypersensitivity effect through modulating p38MAPK pathways,which might be beneficial for immune-related diseases.