| 强力霉素对人胃癌细胞体外增殖的影响 |
| |
| 引用本文： | 谢兆兰,朱尤庆,汤小艳,林小玲. 强力霉素对人胃癌细胞体外增殖的影响[J]. 医学新知杂志, 2006, 16(2): 90-91,94 |
| |
| 作者姓名： | 谢兆兰 朱尤庆 汤小艳 林小玲 |
| |
| 作者单位： | 武汉大学中南医院消化内科,武汉,430071 |
| |
| 摘 要： | 目的研究强力霉素对人胃癌细胞SGC-7901增殖的影响及其机制。方法用MTT法和免疫组织化学法分别检测SGC-7901细胞的增殖及基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)蛋白表达水平。结果强力霉素作用于SGC-7901的抑制效应呈浓度和时间依赖性,SGC-7901经强力霉素处理后MMP-2的表达,不同浓度间有显著性差异(P<0．01)。结论强力霉素对胃癌细胞SGC-7901有抑制增殖的作用,其机制可能与下调MMP-2蛋白表达有关。
|
| 关 键 词： | 强力霉素 基质金属蛋白酶-2(MMP-2) |
| 收稿时间： | 2005-08-03 |
| 修稿时间： | 2005-08-03 |
Effect of Doxycycline on Human Gastric Cancer Cells SGC-7901 Proliferation in Vitro |
| |
| Xie Zhaolan, Zhu Youqing, Tang Xiaoyan, et al. Effect of Doxycycline on Human Gastric Cancer Cells SGC-7901 Proliferation in Vitro[J]. Journal of New Medicine, 2006, 16(2): 90-91,94 |
| |
| Authors: | Xie Zhaolan Zhu Youqing Tang Xiaoyan et al |
| |
| Affiliation: | Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan 430071 |
| |
| Abstract: | Objective To study the effect of doxycycline on human gastric cancer cells SGC-7901 proliferation in vitro and its mechanism. Methods The proliferation of SGC-7901 was detected by MTT assay, the matrix metalloproteinase-2(MMP-2) protein level was measured with immunohistochemistry. Results Doxycycline could inhibit the proliferation of human gastric cancer cells SGC-7901. The rate of inhibition increased with time and dosage dependence. Immunohistochemical results demonstrated that doxycycline could decrease the MMP-2 protein expression(P < 0.01)in a concentration-dependent manner. Conclusion Doxycycline could inhibit the proliferation of SGC-7901, possibly by decreasing MMP-2 protein expression. |
| |
| Keywords: | doxycycline matrix metalloproteinase-2(MMP-2) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
