裂褶菌孢内多糖和孢外多糖对小鼠免疫功能的影响

裂褶菌孢内多糖(SPG₁)和孢外多糖(SPG₂)是分别从发酵培养的裂褶菌菌丝体和发酵液中提取的多糖。SPG₁和SPG₂均能显著促进Con A诱导的小鼠脾淋巴细胞增殖反应,SPG₁还显著地对抗氢可的松对淋巴细胞增殖反应的抑制作用。SPG₁和SPG₂均可恢复14月龄老年小鼠低下的脾淋巴细胞增殖反应。给小鼠ip SPG₁和SPG₂均能显著增强二硝基氯苯(DNCB)所致小鼠迟发型皮肤过敏反应(DCH)。小鼠ip SPG₁可显著增强羊红细胞(SRBC)诱导的小鼠脾脏空斑形成细胞(PFC)反应。SPG₁和SPG₂还可使老年小鼠的PFC反应恢复至成年小鼠水平。

EFFECTS OF THE POLYSACCHARIDES ISOLATED FROM MYCELIUM AND FERMENTATION FLUID OF SCHIZOPHYLLUM COMMUNE ON IMMUNE FUNCTION IN MICE

SPG1 and SPG2 are polysaccharides extracted respectively from fermented cultural mycelium and fermentation fluid of Schizophyllum commune. This paper reports the effect of SPG1 and SPG2 on concanavalin A (Con A) induced proliferation of lymphocyte from C57BL/6J mice in vitro. SPG1 (10, 25, 50 and 100 micrograms/ml) and SPG2 (10, 50 and 100 micrograms/ml) was found to significantly increase lymphocyte proliferation. In addition, SPG1 (10, 25, 50 and 75 micrograms/ml) antagonized the suppressive effect of hydrocortisone on proliferation of mouse spleen cells, while SPG2 did not. In vivo, at the dose of 2.5 and 5.0 mg/kg ip for 9 days, SPG1 and SPG2 remarkably enhanced the delayed-type hypersensitivity to 2, 4-dinitrochlorobenzene (DNCB) in mice. SPG1 (1.25, 2.5 and 5.0 mg/kg, ip for 1 day) and SPG2 (2.5 and 5.0 mg/kg, ip for 1 day) promoted the plaque forming cell (PFC) response to sheep red blood cells (SRBC) in mice. The effects of SPG1 and SPG2 on immune function were studied in aged immunodeficient mice. SPG1 (10 and 25 micrograms/ml) and SPG2 (25 micrograms/ml) was found to enhance the Con A induced lymphocyte proliferation in 14 months aged mice. At the dose of 2.5 mg/kg, SPG1 and SPG2 produced respectively 162.1 and 136.0% increase in PFC response in 14 months aged mice.