乌头类生药中主要生物碱的薄层光密度法测定

乌头为常用中药,有祛风、散寒、止痛的功效。除正品乌头Aconitum carmichaeli Debx和北乌头Aconitum kusnezoffii Reichb外,同属一些其它品种,亦在民间广泛使用。该属植物多含有双酯类生物碱剧毒成分,主要为乌头碱(aconitine)、中乌头碱(mesaconitine)和

DETERMINATION OF THE MAIN ALKALOIDS IN WU TOU (ACONITE) BY TLC-DENSITOMETRY

A method of TLC densitometry was established in order to determine the main alkaloids: mesaconitine, aconitine and hypaconitine in Aconite root. The powdered sample was alkalinized by ammonia and macerated with ether for 24 h. The mixture was then centrifuged, the residue was washed three times each with 2 ml of fresh ether, the combined ether extract was evaporated to dryness and then dissolved in 1 ml of dichloromethane. Standard solution and sample solution were spotted on a sillca gel GF254 plate, and developed with cyclohexane-ethyl acetate-diethylamine (8:1:1), the chromatogram was observed under UV light as dark spots. A Shimazu TLC model 910 was used for scanning at lambda s 236 nm and lambda R 350 nm by reflection mode. Linear calibration curves were obtained for the 3 constituents in the range of 2-6 micrograms. The average recoveries of masaconitine, aconitine and hypaconitine were 97.3, 96.4, 99.1% and the variation coefficients were 1.81, 1.72, 1.18%, respectively. The spots were stable for more than 24 h. Samples from various sources were analyzed.