胺类药物的胶束色谱研究

以十二烷基硫酸钠(SDS)和溴化十六烷基三甲胺(CTAB)两者的胶束水溶液为流动相,利用高效液相色谱法,研究了七种具有典型分配率的胺类药物和有机弱酸的胶束色谱保留机理,以及胶束溶液在不同pH值时对溶质保留值的影响,提出了胶束色谱保留机理的指数模型公式。该公式可用于估计溶质的胶束色谱类型,对溶质与三相(胶束相、表面活性剂修饰的固定相及总水相)的静电作用、疏水作用对溶质保留值的影响做了合理的解释。

MICELLAR CHROMATOGRAPHIC STUDY OF AMINES

Aqueous solutions of the cationic surfactant, hexadecyl-trimethyl ammonium bromide (CTAB), and the anionic surfactant, sodium dodecylsulfate (SDS) were used as mobile phase in HPLC to study the micellar chromatographic retention mechanism of seven amines and weak organic acids with various distribution coefficients and to study the influence of different pH values mobile phase on the solute retention. An exponential model equation for micellar chromatographic retention mechanism was put forward, which gives a reasonable explanation of the effects of electrostatic interaction and hydrophobic interaction between the solute and the three phases (micelle phase, surfatant modified stationary phase and bulk water phase). This equation can be used to estimate the micellar chromatographic retention pattern of the solute.