大肠癌组织中端粒酶活性的研究

目的研究大肠癌组织及相应癌旁组织中的端粒酶活性,探讨其作为肠癌肿瘤标记物的可能性。方法采用ＴＲＡＰ方法研究了４０例大肠组织（包括３７例大肠癌,３例良性大肠疾病）和２０例相应癌旁上皮组织中的端粒酶活性表达。结果２０例癌旁上皮和３例良性疾病组织中,端粒酶活性表达均为阴性;而３７例大肠癌组织中,３５例显示端粒酶活性表达为阳性,阳性率为９４．６％。结论端粒酶是特异性较强的恶性肿瘤基因标志,有可能成为大肠癌早期诊断和治疗的理想标志物

Study of telomerase activity in colorectal carcinomas

Objective To investigate the telomerase activity in human colorectal carcinoma and tumor adjacent tissue and to investigate the possibility of telomerase as tumor biological marker. Methods A total of 60 human colorectal tissue specimens, comprising 37 carcinomas, 3 benign lesions and 20 tumor adjacent tissue specimens were examined for telomerase activity by telomeric repeat amplification protocol(TRAP) assay. Results No telomerase activity in 20 tumor adjacent tissues and 3 benign lesions was detected. However, of 37 colorectal carcinoma specimens, 35 were positive for telomerase activity with a 94.6% positive rate. Conclusion The data suggest that telomerase is a tumor specific gene marker, and may play an important role in early diagnosis and treatment of colorectal carcinomas.