HCV不同功能区抗体与HCVRNA的关系探讨

探讨HCV不同功能区抗体(抗-C、抗-NS3、抗-NS4及抗-NS5)与HCVRNA的关系。HCV不同功能区抗体测定采用ELISA法,用RT-PCR进行HCV RNA检测。结果显示55例抗HCV阳性血清中有7种抗体阳性组合。抗-NS3、抗-C、抗-NS5及抗-NS4在7种阳性组合中的检出率分别为96．4%、89．1%、58．2%、56．4%。HCVRNA阳性血清中抗-NS3、抗-C、抗-NS5及抗NS4的检出率分别为96．2%,88．5%,57．7%,57．7%。HCV不同功能区抗体ELISA法有很高的敏感性和特异性,与RT-PCR法基本一致。抗-NS3、抗-C在HCV的诊断中有重要的意义,抗-NS5和抗-NS4有诊断的互补作用。

Relationship between anti-bodies for different regions of HCV and HCV RNA

To investigate the relationship between anti-bodies for different regions of HCV(anti-c,anti-NS3,anti- NS4 and anti-NS5)and HCV RNA.Different region antibodies of HCV were detected by ELISA,and HCVRNA detected by RT-PCR.There were seven positive groups in 55 cases with anti-HCV positive.The positive rates of anti-NS3,anti -C,anti-NS5 and anti-NS4 in the seven positive group were 96.4%,89.1%,58.2%,and 56.4% respectively.The positive rates of anti-NS3,anti-C,anti-NS5 and anti-NS4 in serum with HCVRNA positive were 96.2%,88.5%, 57.7% and 57.7% separately.Anti-HCV different region ELISA has the same sensitivity and specificity as that of RT- PCR.Anti-NS3 and anti-C were very important to diagnose HCV.anti-NS5 and anti-NS4 had mutually complemen- tary effect in diagnosing HCV.