硝酸甘油对持续缺血和缺血再灌注模型犬心肌细胞凋亡的影响

目的探讨硝酸甘油对持续缺血和缺血再灌注损伤心肌细胞凋亡的影响。方法 取杂种犬30只,随机分为假手术组,持续缺血组,缺血再灌注组,硝酸甘油组和硝酸甘油+持续缺血组。以活结结扎左冠状动脉的前降支,造成持续阻断冠状动脉血流和再灌注。以原位末端标记法标记凋亡细胞。结果 持续缺血组和缺血再灌注组与假手术组比较,心肌细胞凋亡程度明显增加,差异有显著性意义。硝酸甘油组和硝酸甘油+持续缺血组分别与持续缺血组和缺血再灌注组比较,心肌细胞凋亡程度明显降低,差异有显著性意义。结论 持续缺血和缺血再灌注损伤可导致心肌细胞凋亡,硝酸甘油预处理能明显减轻心肌细胞凋亡程度。

The effect of nitroglycerin on the apoptosis of myocardial cells during the continuous ischemia and ischemia-reperfusion injury in the model dog

Objective To study the effect of nitroglycerin on the apoptosis of myocardial cells during the continuous ischemia and the ischemia-reperfusion injury. Methods Dogs were randomly divided into 5 groups. Acute myocardial infarction animal models were formed by ligating the anterior descending branches of the left coronary artery with slipknot to block the blood flow,followed by reperfusing the myocardium. The apoptotic cell was labeled by TUNEL. Results In situ apoptotic cell labeling and semiquantitative analysis showed that compared with sham operation group, the degree of the apoptosis of the myocardial cell was increased significantly in the continuous ischemia group and ischemia-reperfusion group. Compared with continuous ischemia group and ischemia-reperfusion injury group, the degree of the apoptosis of the myocardial cells was reduced significantly in the nitroglyeerin-administrated group. Conclusions Continuous ischemia and ischemia-reperfusion injury could result in the apoptosis of myocardial cells. Pretreatment with nitroglyeerin could lessen the degree of apoptosis of the myocardial cells significantly.