氟喹诺酮类药物与甲氧苄啶联合抗菌作用的实验研究

目的：探讨TMP对氟喹诺酮类药物的增效作用，以及与氟喹诺酮类药物的最佳配伍比例。方法：将菌液制成0．5麦氏比浊标准，采用肉汤稀释法测定细菌对TMP和氟喹诺酮类药物的MIC；按棋盘法设计测定7种氟喹诺酮类药物和TMP联合试验时FIC，判断各药与TMP联合作用效应。结果：选择的7种氟喹诺酮类药物与TMP配伍使用均产生协同作用或相加作用，使各氟喹诺酮类药物抗菌作用增强1～4倍，不同的氟喹诺酮类药物与TMP配伍的最佳比例因药物和受试菌的种类不同而异。结论：氟喹诺酮类药物与TMP联合应用能起协同作用，但TMP对不同的氟喹诺酮类药物的增效作用不完全相同．二者配伍也不一。临床上在配伍使用前府作联合药敏试验．以选择最佳配伍比例。

THE EXPERIMENTAL RESEARCH ON CONSOLIDATED ANTI- BACTERIAL ACTION OF FLUOROQUINOLONFS MEDICINES AND TRIMETHOPRIN

Objective:To probe the effect-enhancement of TMP to fluoroquinolones medicines and the best blend proportion of TMP and fluoroquinolones medicines.Methods:Bacterial fluid of 0.5 McFarland standard and broth dilution method was used to test its MIC to TMP and fluoroquinolones medicines;checkerboard method was also used to determine FIC of seven fluoroquinolones medicines and TMP during consolidated experiments,thus to determine the respective effect.Results:When seven kinds of fluoroquinolones medicines were respectively blended and used with TMP,synergism of addition occured and anti-bacterial action of fluoroquinolones increased by 1-4 times.The best blend proportion of fluoroquinolones medicines and TMP was different because of the different kinds of fluoroquinolones medicines and tested bacteria.Conclusion:The consolidated usage of fluoroquinolones medicines and TMP can cause synergism,but the effect-enhancement of TMP to different fluoroquinolones medicines is not absolutely the same and their blend proportion is also different.In clinical practice consolidated test should be made before blend usage in order to choose the best proportion.