胃癌组织中PCNA和细胞色素C蛋白的表达及其临床意义

目的：研究胃癌组织中增殖细胞核抗原(PCNA)和细胞色素C(CytC)蛋白的表达、相互关系及其临床意义。方法：应用免疫组织化学S-P法，检测59例胃癌组织及10例正常胃黏膜组织中PCNA和Cyt C蛋白的表达。 结果：10例正常胃黏膜组织中，PCNA和Cyt C蛋白表达率分别为20.0%（2/10）和10.0%（1/9），胃癌组织中PCNA和CytC蛋白的表达率分别为86.4%和78.0%，胃癌组织表达率高于正常胃黏膜组织（P<0.05 或P<0.01）。在不同年龄、不同肿瘤大小的胃癌患者中PCNA和CytC的表达率比较差异无显著性（P>0.05），低分化患者PCNA的表达率高于高分化患者（P<0.05），低分化和中分化患者CytC蛋白表达率均低于高分化患者（P<0.01），胃癌组织中PCNA蛋白与Cyt C蛋白的表达频数差异无显著性（P>0.05）。结论：PCNA蛋白的高表达及Cyt C的低表达与胃癌的发生发展有关，提示二者在胃癌的发生和发展过程中分别通过不同途径来发挥生物学作用。

Expressions of PCNA and Cyt C protein in gastric carcinoma tissue and their clinical significances

Objective To explore the expressions of proliferating cell nuclear antigen(PCNA)and cytochrome C(Cyt C)in gastric carcinoma tissue and discuss their relationship and clinical significances.Methods The expressions of PCNA and Cyt C in 59 gastric carcinoma samples and 10 normal gastric mucosa samples were detected by immunohistochemistry.Results In 10 normal gastric mucosa tissue,the positive rates of PCNA and Cyt C were 20.0%(2/10)and 10.0%(1/9),respectively.In gastric carcinoma,the positive rates of PCNA and Cyt C were 86.4% and 78.0%,respectively.The expression levels of PCNA and Cyt C in gastric carcinoma were significantly higher than those in normal gastric mucosa tissue(P<0.05 or P<0.01).The expressions of PCNA and Cyt C had no relationship with either the age of patient or the size of tumor(P>0.05),but had relationship with histological type(P<0.05).The expression levels of PCNA and Cyt-C in poorly differentiated tissue were higher than those in well differentiated tissue(P<0.05),the expression levels of PCNA and Cyt C in poorly differentiated tissue and moderately differentiated tissue were higher than those in well differentiated tissue(P<0.01).In gastric carcinoma tissue,the expressions of PCNA and Cyt C had no difference(P>0.05).Conclusion The high expression of PCNA and low expression of Cyt C are related to the occurrence and development of gastric carcinoma,and they play an important role with different methods.