| 制造病毒性心肌炎动物模型毒株的比较 |
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| 引用本文： | 崔小岱,马连华,许峥. 制造病毒性心肌炎动物模型毒株的比较[J]. 中华儿科杂志, 2000, 38(2): 83-85 |
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| 作者姓名： | 崔小岱 马连华 许峥 |
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| 作者单位： | 首都儿科研究所 |
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| 基金项目： | 北京市科技新星计划资助! (951870700) |
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| 摘 要： | 目的 比较柯萨奇Ｂ３ｍ株病毒（ＣＶＢ３ｍ）和柯萨奇Ｂ３ａ株病毒（ＣＶＢ３ａ）在实验中引起小鼠心肌炎的发病情况，为心肌炎造模者客观的实验参考。方法 用ＣＶＢ３ｍ和ＣＶＢ３ａ感染Ｂａｌｌ３／ｃ实验小鼠，测定两株平素在鼠心肌细胞中的滴度；用常规病毒理法观察两株病毒致小鼠心肌炎的情况。结果ＣＶＢ３ｍ和ＣＶＢ３ａ感染组小鼠心肌病毒滴度的峰值出现的时间基本相同，ＣＶＢ３ｍ的滴度较高；ＣＶＢ３ｍ感染组小鼠死亡率
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| 关 键 词： | 心肌炎 柯萨奇病毒B组 动物模型 |
The comparison of virus strains on establishing the murine viral myocarditis model |
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| CUI Xiaodai,MA Lianhua,XU Zheng,et al.. The comparison of virus strains on establishing the murine viral myocarditis model[J]. Chinese journal of pediatrics, 2000, 38(2): 83-85 |
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| Authors: | CUI Xiaodai MA Lianhua XU Zheng et al. |
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| Affiliation: | CUI Xiaodai,MA Lianhua,XU Zheng,et al. Capital Institute of Pediatrics,Beijing 100020,China |
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| Abstract: | Objective To investigate the development mouse myocarditis induced with coxsackievirus B3m(CVB 3m ) and B3o(CVB 3o ) and provide references to researchers interesting in such murine model. Methods CVB 3m and CVB 3o were used to infect Balb/c mice. The virus titer was detected in the mouse myocardium. The mouse myocarditis was diagnosed with the routine histopathological method. Results The peak titer of CVB 3m in mouse myocardium was higher than that of CVB 3o . The death rate of CVB 3m infected mice was much higher than that of CVB 3o . The incidences of CVB 3m and CVB 3o induced murine myocarditis were 76% and 17%, respectively. Conclusion There were obvious differences between CVB 3m and CVB 3o on inducing murine myocarditis. CVB 3m was suggested to establish the murine myoocarditis model. |
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| Keywords: | Myocarditis Disease models aninal Coxsackieviruses B |
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