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板蓝根药材的HPLC特征指纹图谱研究
引用本文:陈勇,杨文清,蔡敏,杜静,韩凤梅. 板蓝根药材的HPLC特征指纹图谱研究[J]. 中医药学报, 2003, 31(3): 18-19
作者姓名:陈勇  杨文清  蔡敏  杜静  韩凤梅
作者单位:湖北大学生命科学学院,湖北,武汉,430062
基金项目:武汉市科技局重点攻关项目 (2 0 0 1 0 1 0 1 0 0 8- 5),科技部十五攻关项目 (2 0 0 1BA70 1A0 1 )
摘    要:目的:研制板蓝根药材HPLC特征指纹图谱。方法:应用BP—HPLC法分离分析板蓝根对照药材水提取物色谱图,并以5种市售板蓝根药材的色谱图做验证,建立板蓝根药材指纹图谱。结果:不同来源板蓝根药材的水提取物色谱图基本相同,特征共有峰有8个,但相对谱峰面积因药材来源不同存在一定差异。结论:所建立的方法用于板蓝根药材鉴别与质量评价简便、准确,并为建立板蓝根药材HPLC指纹图谱提供了实验依据。

关 键 词:板蓝根 中药鉴别 HPLC 指纹图谱
文章编号:1002-2392(2003)03-0018-02

Research on Character Spectra of Radix Isatidis By HPLC
CHEN Yong,YANG Wen-qing,CAI Min,et al. Research on Character Spectra of Radix Isatidis By HPLC[J]. Acta Chinese Medicine and Pharmacology, 2003, 31(3): 18-19
Authors:CHEN Yong  YANG Wen-qing  CAI Min  et al
Abstract:Objective:The HPLC character spectra of Radix Isatidis was studied and established.Method:The water-soluble extraction of Radix Isatidis standard herba studied by RP-HPLC to establish the character spectra,which was verificated by the water-soluble extractions of Radix Isatidis from five different kinds of sources.Result:The peaks were almost the same,there being 8 common peaks,while the relative area of the common peaks of different samples varied from different origins.Conclusion:The experimental results could serve as the convenient and effective identification and quality evaluation,which also offered testimony for the establisment of HPLC-FPS of Radix Isatidis. Author's address:Acedemy of Life Science,Huibei University,Wuhan 430062,China
Keywords:Radix Isatidis  HPLC  HPLC-FPS  
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