Purification of biologically active rubella virus antigens by immunoaffinity chromatography |
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Authors: | P Chong S Gillam |
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Affiliation: | Department of Pathology, Faculty of Medicine, University of British Columbia, Vancouver, B.C., Canada, V6T 1W5 |
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Abstract: | A general procedure for isolating biologically active rubella virus antigens (VPI, Mr = 61,000; VP2, Mr = 45,000; VP3, Mr = 36,000) by monoclonal antibody affinity chromatography is described. Complexes formed between monoclonal antibodies and rubella virus antigens were found to be stable either at low pH or in Tris buffer containing detergent and high salt, but were efficiently dissociated by 5% diethanolamine, pH 11.5, or 50 mM lithium diiodosalicylate buffer, pH 8.0. Chromatographically purified rubella viral antigens retained their antigenicity as determined by enzyme-linked immunosorbent assays. Biological studies showed that rubella structural proteins VP2 and VP3 had no hemagglutinin function while the mixture of VP1 and VP2 and VP3 directly demonstrated hemagglutination activity. These results indicate that VP1 is at least in part responsible for the hemagglutinin function of rubella virus. |
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Keywords: | rubella virus affinity chromatography hemagglutination rubella virus antigens MAb monoclonal antibody MAb-Sepharose 4B monoclonal antibody covalently bound to Sepharose 4B ELISA enzyme-linked immunosorbent assay HA hemagglutination activity SDS- PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis PBS phosphate-buffered saline |
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