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MBL对树突状细胞体外分化成熟的影响
引用本文:陈月,陈政良,左大明,裘毅刚,张丽芸. MBL对树突状细胞体外分化成熟的影响[J]. 细胞与分子免疫学杂志, 2005, 21(2): 159-162
作者姓名:陈月  陈政良  左大明  裘毅刚  张丽芸
作者单位:南方医科大学基础部免疫学教研室,广东,广州,510515
摘    要:目的: 探讨甘露聚糖结合凝集素 (MBL)对人外周血单核细胞来源的树突状细胞 (MoDC)分化成熟的影响。方法: 以天然人MBL刺激MoDC, 在倒置显微镜下观察DC的形态; 用FACS分析DC的表型; 用 3H- TdR掺入法测定DC刺激同种异体T细胞增殖的能力; 以酵母多糖颗粒吞噬试验评估DC的抗原摄取能力; 用ELISA检测DC培养上清中IL- 12和TNF- α的含量。结果: MBL刺激的DC表面分子CD1a、CD83、CD40、CD80、CD86和MHC DR的表达均上调,摄取酵母多糖颗粒的能力降低, 激发初始T细胞增殖的能力加强, 分泌的IL- 12增多但几乎不分泌TNF- α。结论: MBL能诱导DC分化成熟, 提示其可能通过调节DC的功能而参与获得性免疫应答。

关 键 词:甘露聚糖结合凝集素  树突状细胞  分化  天然免疫
文章编号:1007-8738(2005)02-0159-04
修稿时间:2004-07-28

In vitro effect on the differentiation and maturation of monocyte-derived dendritic cells by mannan-binding lectin
CHEN Yue,Chen Zheng-liang,ZUO Da-ming,QIU Yi-gang,ZHANG Li-yun. In vitro effect on the differentiation and maturation of monocyte-derived dendritic cells by mannan-binding lectin[J]. Chinese journal of cellular and molecular immunology, 2005, 21(2): 159-162
Authors:CHEN Yue  Chen Zheng-liang  ZUO Da-ming  QIU Yi-gang  ZHANG Li-yun
Affiliation:Department of Immunology, Southern Medical University, Guangzhou 510515, China. chenyue@fimmu.com
Abstract:AIM: To explore the effect of mannan-binding lectin (MBL) on the differentiation and maturation of human peripheral blood monocyte-derived dendritic cells (MoDCs). METHODS: After MoDCs was stimulated with human natural MBL, MoDC's morphology was observed under inverted microscope and the expressions of CD1a, CD83, CD40, CD80, CD86 and HLA-DR on MoDCs were analyzed by FACS. The ability of MoDCs to stimulate the proliferation of allogenic T cells was detected by (3)H-TdR incorporation. The ability of MoDCs to up-take antigens was evaluated by zymosan granule phagocytosis test. The levels of IL-12 and TNF-alpha in the culture supernatant of MoDCs were determined by ELISA. RESULTS: The expressions of CD1a, CD83, CD40, CD80, CD86 and HLA-DR on the MoDCs were up-regulated by MBL. The ability of MoDCs to up-take zymosan granules decreased and the proliferation of native T cells induced by MoDCs was enhanced. MBL stimulated the production of IL-12 by MoDCs, but had no such effect on TNF-alpha secretion. CONCLUSION: MBL can induce differentiation and maturation of DCs in vitro, suggesting that MBL possibly participate in the adaptive immune response through modulation of functions of DCs.
Keywords:mannan-binding lectin  dendritic cells  differentiation  innate immunity
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