| 双黄连注射液对致敏RBL-2H3细胞组胺释放的影响 |
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| 引用本文： | 王青,罗霞,周联,董燕.双黄连注射液对致敏RBL-2H3细胞组胺释放的影响[J].深圳中西医结合杂志,2011,21(4):197-199. |
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| 作者姓名： | 王青 罗霞 周联 董燕 |
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| 作者单位： | 广州中医药大学临床药理所免疫研究室,广东广州,510405 |
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| 基金项目： | 广东高校优秀青年创新人才培育项目(LYM09049); 广东省中医药管理局科研课题(2009225) |
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| 摘 要： | 目的：建立致敏RBL-2H3细胞模型,观察双黄连注射液对其组胺释放的影响,探讨该模型用于体外评价中药注射剂致敏性的可行性。方法：双黄连注射液联合弗氏佐剂免疫Wistar大鼠,收集致敏后大鼠血清,将其与体外培养RBL-2H3细胞孵育12h,建立致敏RBL-2H3细胞模型,双黄连注射液与致敏RBL-2H3细胞共培养1.5h,ELISA法检测培养上清中组胺含量。结果：双黄连注射液致敏大鼠血清IgE水平明显升高,与对照组相比P〈0.05,差异有统计学意义。组胺实验结果显示,双黄连注射液可显著引起致敏RBL-2H3细胞组胺释放,与对照组相比P〈0.05,差异有统计学意义。结论：双黄连注射液可促进致敏RBL-2H3细胞释放组胺,提示RBL-2H3细胞模型具有评价中药注射液所致过敏反应的潜在应用价值
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| 关 键 词： | 双黄连注射剂 RBL-2H3细胞 组胺 |
Effects of Shuanghuanglian Injection on the Histamine Release of Sensitized RBL-2H3 Cells |
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| WANG Qing,LUO Xia,ZHOU Lian,DONG Yan.Effects of Shuanghuanglian Injection on the Histamine Release of Sensitized RBL-2H3 Cells[J].Shenzhen Journal of Integrated Traditional Chinese and Western Medicine,2011,21(4):197-199. |
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| Authors: | WANG Qing LUO Xia ZHOU Lian DONG Yan |
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| Affiliation: | WANG Qing,LUO Xia,ZHOU Lian,DONG Yan(Department of Immunology,Guangzhou University of TCM,Guangdong Guangzhou 510405) |
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| Abstract: | Objective To establish the sensitized RBL-2H3 cell models,study the effect of Shuanghuanglian Injections on the release rate of histamine and explore the feasibility of evaluating traditional Chinese medicine injections-induced anaphylaxis reaction by RBL-2H3 cell model.Methods Wistar rat was sensitized with Shuanghuanglian injections combined with Freund's adjuvant and the blood serum was collected,then RBL-2H3 cell were cultured with the blood serum for one and a half hours.The content of histamine in sup... |
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| Keywords: | Shuanghuanglian Injections RBL-2H3 cells Histamine |
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