| X线照射后对乳腺癌细胞凋亡的影响及CDKN1A表达的变化 |
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| 引用本文： | 高炳玉,夏立平,刘玉,陈国平,郑武平.X线照射后对乳腺癌细胞凋亡的影响及CDKN1A表达的变化[J].肿瘤防治研究,2011,38(8):891-894. |
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| 作者姓名： | 高炳玉 夏立平 刘玉 陈国平 郑武平 |
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| 作者单位： | 570102 海口，海南医学院附属医院肿瘤科 |
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| 摘 要： | 目的探讨乳腺癌细胞系（MCF-7）X线照射后CDKN1A基因（p21）表达变化及其对细胞凋亡的影响。方法用流式细胞方法检测MCF-7细胞在接受不同剂量X射线照射后CDKN1A基因表达的改变和用RNAi技术抑制CDKN1A基因表达并检测细胞凋亡的变化。结果MCF-7细胞在接受不同剂量（1、2和4 Gy） X射线照射后CDKN1A蛋白表达有不同程度升高，其中 4 Gy照射后升高水平最明显（P<0.05）。在接受4 Gy剂量照射后，CDKN1A蛋白水平在8、12、24、48、72 h 均有不同程度增加，其中24 h时较对照组升高3倍(P<0.05)。抑制CDKN1A基因表达后MCF-7细胞凋亡率增加183.9%（P<0.05）。结论乳腺癌细胞在接受4 Gy照射后24 h的CDKN1A表达水平增加最为明显，抑制CDKN1A基因表达可促进细胞凋亡。
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| 关 键 词： | RNA干扰 CDKN1A基因 电离辐射 细胞凋亡 |
| 收稿时间： | 2011-05-03; |
Effection of Apoptosis on Breast Cancer Cell and Changes of CDKN1A Gene Expression after X-ray Radiation |
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| GAO Bing-yu,XIA Li-ping,LIU Yu,CHEN Guo-ping,ZHENG Wu-ping.Effection of Apoptosis on Breast Cancer Cell and Changes of CDKN1A Gene Expression after X-ray Radiation[J].Cancer Research on Prevention and Treatment,2011,38(8):891-894. |
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| Authors: | GAO Bing-yu XIA Li-ping LIU Yu CHEN Guo-ping ZHENG Wu-ping |
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| Institution: | Department of Oncology, The Affiliated Hospital of Medcial College, Haikou 570102， China |
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| Abstract: | Objective To study the changes of expression of CDKN1A gene(p21) after the X-ray radiation on breast cancer cell line(MCF-7) and the effect of expression of CDKN1A on cellular apoptosis.Methods Expression of CDKN1A gene in MCF-7 cells was detected at different time and radiation dose by flow cytometric test.Cellular apoptosis was analyzed under CDKN1A gene inhibition by RNA interfference(RNAi).Results After the X-ray irradiation on MCF-7 cells with 1,2 and 4 Gy,the expression of CDKN1A protein increased gra... |
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| Keywords: | RNA interference CDKN1A gene Radiation Apoptosis |
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