| 亚胺培南耐药鲍曼不动杆菌由插入序列ISAba1点突变引起的bla_(OXA-23)表达减低 |
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| 引用本文： | 武大伟,魏殿军,马全玲,门昆,曹阳. 亚胺培南耐药鲍曼不动杆菌由插入序列ISAba1点突变引起的bla_(OXA-23)表达减低[J]. 中国抗生素杂志, 2011, 0(5) |
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| 作者姓名： | 武大伟 魏殿军 马全玲 门昆 曹阳 |
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| 作者单位： | 天津医科大学第二医院检验科; |
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| 基金项目： | 天津市卫生局科技基金资助项目(06KZ31); 天津市高等学校科技发展基金计划项目(20060620) |
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| 摘 要： | 目的研究临床分离的8株亚胺培南耐药鲍曼不动杆菌中,与插入序列相关的blaOXA-23 mRNA的表达情况。方法应用双纸片增效法进行金属酶表型筛查并以PCR的方法扩增blaIMP-1、blaIMP-2、blaVIM-2和blaOXA-23 4种β-内酰胺酶基因;实时荧光PCR检测blaOXA-23 mRNA表达量,并以PCR的方法扩增插入序列ISAba1并测序。结果 4株菌金属酶表型筛查为阳性结果,PCR检测8株菌blaOXA-23阳性,2株blaIMP-1阳性、3株blaIMP-2阳性、blaVIM-2基因均为阴性;所有菌株均存在插入序列ISAba1,实时荧光PCR检测显示1株菌blaOXA-23 mRNA表达升高,其余7株菌与对照株比较为表达减低或相近,且这7株菌的插入序列存在点突变现象。结论插入序列ISAba1点突变是引起blaOXA-23碳青霉稀酶活性减低的主要原因。
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| 关 键 词： | 鲍曼不动杆菌 blaOXA-23mRNA 插入序列ISAba1 |
Study on lower expression of bla_(OXA-23) associated with point mutation of insertion sequence ISAba1 in imipenem-resistance Acinetobacer baumannii |
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| Wu Da-wei,Wei Dian-jun,Ma Quan-ling,Men Kun , Cao Yang. Study on lower expression of bla_(OXA-23) associated with point mutation of insertion sequence ISAba1 in imipenem-resistance Acinetobacer baumannii[J]. Chinese Journal of Antibiotics, 2011, 0(5) |
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| Authors: | Wu Da-wei Wei Dian-jun Ma Quan-ling Men Kun Cao Yang |
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| Affiliation: | Wu Da-wei,Wei Dian-jun,Ma Quan-ling,Men Kun and Cao Yang(Department of Laboratory Medicine,the Second Hospital,Tianjin Medical University,Tianjin 300211) |
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| Abstract: | Objective To investigate the expression of blaOXA-23 mRNA in imipenem-resistance strains.Methods MBLs were checked by imipenem/imipenem-EDTA combination test;blaIMP-1,blaIMP-2,blaVIM-2 and blaOXA-23 encoding genes were detected by PCR;blaOXA-23 mRNA expression was detected by real-time RT RCR,insertion gene ISAba1 was amplified by PCR,then was sequenced.Results 4 strains showed MBLs positive;8 strains blaOXA-23 positive,2 strains blaIMP-1 positive,3 strains blaIMP-2 positive,blaVIM-2 genes were negative;1 s... |
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| Keywords: | Acinetobacter baumannii blaOXA-23 carbapenemase Insert sequence ISAba1 |
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