| 解吸方法对头孢菌素C提取质量的影响 |
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| 引用本文： | 唐敦武,陈光,何建勇.解吸方法对头孢菌素C提取质量的影响[J].沈阳药科大学学报,2007,24(10):607-610,647. |
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| 作者姓名： | 唐敦武 陈光 何建勇 |
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| 作者单位： | 沈阳药科大学,制药工程学院,辽宁,沈阳,110016 |
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| 摘 要： | 目的研究不同的解吸方法对头孢菌素C提取质量的影响。方法将头孢菌素C发酵上清液通过XAD16大孔吸附树脂进行吸附,然后分别采用单一浓度解吸(均一解吸)及梯度浓度解吸的方法进行解吸。结果梯度浓度解吸与单一浓度解吸相比,解吸液的纯度提高3.23%,解吸收率提高2.3%。结论梯度浓度解吸法比单一浓度解吸法具有更多的优势,值得推广和应用。
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| 关 键 词： | 头孢菌素C 解吸 分离 提取 大孔吸附树脂 |
| 文章编号： | 1006-2858（2007）10-0607-04 |
| 修稿时间： | 2007-01-29 |
Effects of different methods of elution on extraction quality of cephalosporin C |
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| TANG Dun-wu,CHEN Guang,HE Jian-yong.Effects of different methods of elution on extraction quality of cephalosporin C[J].Journal of Shenyang Pharmaceutical University,2007,24(10):607-610,647. |
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| Authors: | TANG Dun-wu CHEN Guang HE Jian-yong |
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| Institution: | School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China |
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| Abstract: | Objective To investigate the effects of different methods of elution on the extraction quality of cephalosporin C.Methods Cephalosporin C was absorbed when the fermentation filtrate of cephalosporin C was passed through a resin column of macro-porous adsorption resin XAD-16,and eluted by uniformity elution method and gradient elution method respectively.Results When gradient elution method was adopted,the purity of cephalosporin C in the eluent was 3.23% higher and the recovery rate of cephalosporin C in the eluent was 2.3% higher than that of uniformity elution method.Conclusions Gradient elution method is worthy of widely use in cephalosporin C production for its advantages compared with uniformity elution method. |
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| Keywords: | cephalosporin C elution separation extraction macro-porous adsorption resin |
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