大豆异黄酮对初老大鼠卵巢Bcl-2基因和Caspase-3基因mRNA表达的影响

目的观察大豆异黄酮(soy isoflavones,SI)对初老大鼠卵巢Bcl-2、Caspas e-3 mRNA表达及氧自由基含量的影响。方法利用自然衰老的雌性大鼠建立初老动物模型,连续灌胃给药8周后,采用逆转录-聚合酶链反应(RT-PCR)检测卵巢Bcl-2和Caspase-3 mRNA表达,采用硫代巴比妥酸和黄嘌呤氧化法分别检测卵巢丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果与初老组相比,各SI处理组卵巢Bcl-2 mRNA表达量和SOD的活性均明显增高、Caspase-3表达量明显下降,中剂量(158 mg.kg-1)和高剂量(500 mg.kg-1)SI处理组MDA含量也显著降低(P<0.05或P<0.01)。结论大豆异黄酮上调卵巢Bcl-2、下调Caspase-3表达、抑制氧自由基生成并增强SOD活性,可能是其改善衰老卵巢功能的机制之一。

Effects of soy isoflavones on the expression of Bcl-2 and caspase-3 mRNA in ovaries of praesenilis famale rats

Objective To investigate the effects of soy isoflavones(SI)on the expression of Bcl-2 and Caspase-3 mRNA and the content of the oxygen free radicals in ovaries of praesenilis famale rats.Methods The animal models of praesenilis rats were established by unforced ageing.The expression of Bcl-2 and Caspase-3 mRNA in ovaries were detected by RT-PCR.The MDA content and SOD activity in ovaries were detected by the methods of thio-malonylurea and zanthine hydrocarbonylation,respectively.Results Intervention of SI could significantly increase the expression of Bcl-2 mRNA and SOD activity in ovaries of praesenilis rats and decrease the expression of caspase-3 mRNA in ovaries of praesenilis rats.The MDA contents of aged ovaries were significantly decreased by administration of 158 mg·kg-1 and 500 mg·kg-1 of SI.Conclusions The reasons that soy isoflavones can improve the functions of aged ovaries in praesenilis rats are partially due to the up-regulation of the Bcl-2 mRNA expression,the down-regulation of the Caspase-3 mRNA expression,the decrease of the production of the oxygen free-radicals,which can induce the apoptosis of aged ovaries,and the increase of the SOD activity.