光动力疗法对人宫颈癌Caski细胞裸鼠移植瘤MIF表达的影响

目的:探讨光动力疗法对人宫颈癌Caski细胞裸鼠移植瘤巨噬细胞移动抑制因子(MIF)表达的影响。方法:选取BALB/c裸鼠,皮下注射人宫颈癌Caski细胞,10d后将达到荷瘤标准的40只裸鼠采用随机数字表法分为四组,即A组、B组、C组和D组,各10只。A组作为阴性对照、B组给予单次光动力疗法、C组给予多次光动力疗法和D组给予顺铂腹腔注射。对比治疗前和治疗2周后肿瘤体积,治疗前后裸鼠体质量、瘤体积、瘤质量变化及抑瘤率,并分别采用western-blot和免疫组化法对各组裸鼠肿瘤组织MIF表达情况进行检测,行对比分析。结果:B组、C组和D组体质量、瘤体积和瘤质量变化和A组比较差异均有统计学意义(P<0.05),且C组和D组上述指标及抑瘤率和B组比较差异均有统计学意义(P<0.05),而C组和D组间各项指标差异均无统计学意义(P>0.05);电镜分析结果显示各组肿瘤胞浆可见明显空泡,团间有明显坏死灶,并且随着光动力疗法剂量增高,坏死区域增大越明显。B组、C组和D组MIF蛋白IOD值均较A组显著降低(P<0.05),且C组和D组MIF蛋白相对表达量和IOD值均较B组显著降低(P<0.05),而C组和D组MIF蛋白相对表达量和IOD值比较差异均无统计学意义(P>0.05)。结论:采用多次光动力疗法对人宫颈癌Caski细胞裸鼠移植瘤效果和顺铂相近,且可显著降低MIF表达水平。

Effect of photodynamic therapy on the expression of MIF of transplanted tumor in human cervical carcinoma Caski cells in nude mice

Objectives:To discuss the effect of photodynamic therapy on the expression of macrophage migration inhibitory factor (MIF) of transplanted tumor in human cervical carcinoma Caski cells in nude mice.Methods:Nude mice of BALB/c were selected and given human cervical carcinoma Caski cells by subcutaneous injection,and the forty mice bearing tmnor criteria after 10d were divided into four groups according to the random digital table method,namely group A,B,C and D,with ten in each.The group A were chosen as the negative control,while the group B were given photodynamic therapy once,the group C were given photodynamic therapy for 3 times and the group D were given cisplatin for intraperitoneal injection.The tumor volume before treatment and at 2 weeks after treatment was compared,and the changes in body mass of nude mice,tumor volume and tumor mass and inhibition rates before and after treatment were compared,and the expressions of MIF of tumor tissues were detected by western-blot and immunohistochemical method and compared and analyzed.Results:The differences in the changes of body mass of nude mice,tumor volume and tumor mass between group B,C and D and group A were statistically significant (P ＜ 0.05),and the differences in the above indexes and the tumor inhibition rates between group C and D and group B were statistically significant (P ＜ 0.05),while that between group C and D was not statistically significant (P ＞ 0.05).The electron microscopic analysis showed that there were obvious vacuoles in the cytoplasm of the tumor cells in each group,and there were obvious necrosis foci in the clusters,and with the increase of the dose of photodynamic therapy,the more obvious the area of necrosis was.The MIF protein IOD values in the group B,C and D were significantly decreased compared with that in the group B (P ＜ 0.05),and the relative expression of protein MIF and IOD values in the group C and D were significantly decreased compared with those in the group B (P ＜ 0.05),while the differences in the relative expression of protein MIF and IOD values between the group C and group D were not statistically significant (P ＞ 0.05).Conclusion:The treatment effect of multiple photodynamic therapy on human cervical cancer Caski cells in nude mice is similar to that of cisplatin,and the expression level of MIF is significantly decreased.