白细胞精子症与宫腔内人工授精周期临床妊娠率的关系

目的:探讨白细胞精子症对行宫腔内人工授精(intrauterine insemination,IUI)后周期临床妊娠率的影响。方法:分析在本中心行IUI男性患者的精液检查结果,根据WHO推荐方法对185例男性患者的精液质量进行分析,采用联苯胺染色法检测精液白细胞浓度,并将患者分为白细胞精子症不育患者组(白细胞浓度>1×10~6/m L,n=33)和精液白细胞正常不育患者组(白细胞浓度≤1×10~6/m L,n=152)。比较两组间的精液量、精子浓度、精子正常形态率、处理前后前向运动精子总数、精子DNA碎片指数、周期临床妊娠率等方面的差异。结果:白细胞精子症不育组行IUI共47个周期,白细胞正常不育组行IUI共279个周期。两组间精液量、精子浓度、处理前后前向运动精子总数无显著性差异(P>0.05)。在精子正常形态率方面,白细胞精子症患者不育组低于白细胞正常不育患者组,有统计学意义(P<0.05)。精子DNA碎片指数方面,白细胞精子症患者不育组临床妊娠率高于白细胞正常不育患者组,有统计学意义(P<0.05)。但是,在临床妊娠率数据中,我们发现白细胞精子症患者不育组与白细胞正常不育患者组无显著差异(P>0.05)。结论:虽然白细胞精子症会影响精子的正常形态、精子存活率以及DNA碎片指数,但是白细胞精子症不影响IUI临床妊娠率。

Relationship between eukocytospermia and clinical pregnancy rate of intrauterine insemination

Objectives:To explore the relationship between leukocytospermia and clinical pregnancy rate of intrauterine insemination (IUI).Methods:185 IUI patients from our center were selected for the retrospective analysis.According to WHO standard method,185 patients were divided into two groups based on the results of Benzedrine staining method,leukocytospermia infertility group (leukocyte number ≥1 × 106/ml) and normal infertility group (leukocyte number ＜ 1 × 106/ml).The semen volume,sperm concentration,sperm morphology,sperm DNA fragment and clinical pregnancy rate of two groups were compared.Results:There were 47 cycles of IUI in leukocytospermia infertility group and 279 cycles of IUI in normal infertility group.There was no significant difference between the two groups in semen volume,sperm concentration,and total number of forward movement sperm before and after treatment (P ＞ 0.05).The rate of normal sperm morphology in leukocytespermia infertility group was lower than that of normal infertility group (P ＜ 0.05).The perm DNA fragmentation index in leukocytespermia infertility group was much higher than that in normal infertility group (P ＜ 0.05).However,the clinical pregnancy rate between two groups had no significant difference (P ＞ 0.05).Conclusion:Leukocytespermia would affect the normal sperm morphology and DNA fragmentation index,but leukocytespermia wouldn't reduce the clinical pregnancy rate of IUI.