磷脂酰肌醇3激酶-蛋白激酶B-叉头框蛋白信号通路在盆腔脏器脱垂中的表达

目的探讨磷脂酰肌醇3激酶(phosphatidylinositol 3 kinase,PI3K)-蛋白激酶B(protein kinase B,Akt)-叉头框蛋白(forkhead box O1,FOXO1)信号通路在盆腔脏器脱垂(pelvic organ prolapse,POP)中的表达及其在POP中的发病机制。方法选取2013年1月至2015年1月因POPⅢ度、Ⅳ度于武汉大学人民医院行全子宫切除术的患者20例(POP组)和同期因其他良性妇科疾病行全子宫切除术的患者20例(对照组),采用蛋白质免疫印记检测骶韧带组织中Akt、磷酸化蛋白激酶B(phosphorylation protein kinase B,p-Akt)、FOXO1、磷酸化(phosphorylation,p-)FOXO1、谷胱甘肽过氧化物酶(glutathione peroxidase1,GPX1)、锰超氧化物歧化酶(manganesesuperoxidedismutase,Mn-SOD)蛋白表达情况。结果 POP组较对照组,骶韧带组织中p-Akt/Akt、p-FOXO1/FOXO1表达比例明显上调,抗氧化蛋白GPX1、Mn-SOD表达下调。结论POP组织中PI3K-Akt-FOXO1信号通路被激活,并下调抗氧化蛋白GPX1、Mn-SOD表达,这一分子机制可能参与POP发生发展。

Expressions of PI3K-Akt-FOXO1 signaling pathwayin pelvic organ prolapse

Objective To investigate the expressions of phosphatidylinositol 3 kinase-protein kinase B-forkhead box O1 (PI3K-Akt-FOXO1) signaling pathway and its pathogenesis in pelvic organ prolapse (POP).Methods 20 patients who underwent total hysterectomy from January 2013 to January 2015 for POP Ⅲ and Ⅳ degrees (POP group) and the 20 patients had total hysterectomy due to other benign gynecological diseases (control group) at Renmin Hospital of Wuhan University were selected.Using protein immunoblotting to detect the expression of Akt, phosphorylation protein kinase B(p-Akt), FOXO1, phosphorylation-FOXO1(p-FOXO1), glutathione peroxidase1(GPX1), manganesesuperoxidedismutase(Mn-SOD) in sacrotic ligament tissue.Results The results showed a significantly higher expression of p-Akt /Akt and p-FOXO1/ FOXO1,but lower expressions of GPX1 and Mn-SOD in the human sacral ligament of the POP group compared to the control group.Conclusion The PI3K-Akt-FOXO1 signaling pathway was activated in the POP tissue and down-regulated the expression of antioxidant protein GPX1 and Mn-SOD, which may be involved in the development and development of POP.