电针“足三里”对酸化乙醇致胃黏膜损伤大鼠的胃组织总NOS、iNOS影响的时效关系研究

目的 :通过电针“足三里”对酸化乙醇致大鼠胃黏膜损伤模型胃黏膜影响的实验 ,探讨针刺的时效关系。方法 :将动物随机分组、造模 ,造模成功后针刺组进行电针。在电针后 7个不同时刻各组进行取材 ,检测胃组织总NOS、iNOS ,将各针刺组与其对应模型组的实验结果进行两两比较。结果 :针刺组与对应时间模型组动物指标均值有一定变化趋势 ,其中针刺 0组比其对应模型 0组总NOS、iNOS活性相对增高。结论 :本实验各针刺组治疗 1次后 ,不同的时段对损伤的胃组织总NOS、iNOS活性的影响不同 ,针刺后即刻可在一定程度上提高胃组织总NOS、iNOS活性 ,因而可能对胃黏膜起一定的保护作用。

Effects of Electroacupuncture of "Zusanli"(ST 36) at Different Time on Gastric Total NOS and iNOS Activity in Alcohol-induced Gastric Mucosal Injury Rats

Objective: To explore time-effect relation of ele ctro-acupuncture (EA) of “Zusa nli”(ST 36) in influencing gastric mucosal total NOS and iNOS activity in rats with a lcohol-induced gastric mucosal injury. Methods: 126 Wistar ra ts were randomly div ided into model control group and EA group which were further divided into 0-h , 0.5-h, 1.0-h, 3.0-h, 6.0-h, 12.0-h, and 24.0-h subgroups (each n =9) respectively . Bilateral “Zusanli” (ST 36) were punctured and stimulated electrically with parameters of frequency of 15 Hz, continuous waves, electric current of 2～3 mA and duration of 30 min. After treatment, the rats of two groups were killed at 0-h, 0.5-h, 1.0-h, 3.0-h, 6.0-h, 12.0-h, and 24.0-h respectively for taking out gastr ic tissues. The contents of total NOS and iNOS in the rats' gastric mucosal tissues were assayed with nitrate reductase method. Results: Comparison between model and EA groups at the aforementioned time courses showed no statistical differe nces in the contents of both total NOS and iNOS of the gastric mucosal tissue ( P>0.05) . Nevertheless, the contents of both total NOS and iNOS in EA-0-h subgroup w ere higher than those of model control-0-h subgroup, suggesting that acupuncture stimula tio n might play an immediate role in raising total NOS and iNOS contents in the gas tric m ucosal tissues to a certain degree. Conclusion: EA of “Zusanl i”(ST 36) may pro duce an instant light action in raising total NOS and iNOS levels in the gastric muco sal tissue, and the time-effect of electroacupuncture in protecting gastric muc osal injury needs to be confirmed further.