赫赛汀对鼻咽癌细胞株体内外生长的影响

 目的 探讨赫赛汀（Herceptin）对鼻咽癌细胞株体内外生长的影响. 方法 采用免疫组化技术检测鼻咽癌组织HER-2/neu基因表达;采用3H-TdR细胞掺入法测定Herceptin对鼻咽癌细胞株体外生长的影响;用荷瘤裸鼠模型观察Herceptin对鼻咽癌细胞株体内生长的影响. 结果 HER-2/neu基因在鼻咽癌组织中有过表达; Herceptin可抑制HER-2/neu基因过表达的鼻咽癌细胞株CNE-2Z 3H-TdR掺入和影响荷瘤裸鼠肿瘤的生长. 结论 Herceptin可抑制CNE-2Z细胞株体外3H-TdR掺入,并影响CNE-2Z细胞株体内的生长.

The Effect of Herceptin on Nasopharyngeal Carcinoma Cells Line Growth in Vivo and in Vitro

Objective To study the effect of Herceptin on nasopharyngeal carcinoma (NPC) cells line growth in vivo and vitro. Methods Immounhistochemical assay was used to detect HER-2/neu gene expression in NPC specimens;the effect of Herceptin on NPC cells line growth were tested by ~3H-TdR incorporation in vivo and by model of xenografts tumor nude mice in vitro. Results HER-2/neu gene overexpression was detected in NPC tissues; the ~3H-TdR incorporation of a NPC cells line CNE-2Z overexpressed HER-2/neu gene was inhibited by Herceptin and Herceptin had the effect of tumor growth on xenografts tumor nude mice. Conclusion The ~3H-TdR incorporation of NPC cells line CNE-2Z was inhibited in vitro and the growth of CNE-2Z had been affected in vivo by Herceptin.