二乙酰二脱水卫矛醇对白血病L1210细胞生长的抑制作用

目的观察二乙酰二脱水卫矛醇(diacetyldianhydrogalactitol,DADAG)对小鼠白血病细胞株L1210细胞的作用,并初步探讨其杀伤肿瘤细胞的机制。方法采用MTT法和平板集落形成法检测细胞的生长情况;3H]-TdR掺入法检测DADAG对L1210细胞DNA合成的影响。结果L1210细胞经不同浓度的DADAG(12.0、17.2、24.5、35.05、0.0 mg.L-1)处理72 h后,其存活率与对照组相比,分别降至72%6、3%、46%3、5%和20%。平板集落形成实验显示DADAG半数集落形成抑制浓度为18.5 mg.L-1。DADAG 12.0、17.2、24.5、35.0、50.0 mg.L-1作用L1210细胞8 h后的3H]-TdR掺入率分别为93.6%、83.9%、42.6%、10.1%和5.7%。结论DADAG对L1210细胞的抑制作用与抑制该细胞DNA合成有关。

Inhibitory effect of diacetyldianhydrogalactitol on leukemic L1210 cells

Objective To observe the effect of diacetyldianhydrogalactitol(DADAG) on mouse L1210 leukemia and investigate the mechanism of cell death induced by DADAG.Methods The growth of cells was examined with MTT assay and colony forming assay.-TdR incorporation into DNA was used to detect the influence of DADAG on DNA synthesis of L1210 cells.Results After incubation of L1210 cells with 12.0,17.2,24.5,35.0 and 50.0 mg·L~(-1) DADAG for 72 h,the survival rate,compared with control group,progressively reduced to 72 %,63 %,46 %,35 % and 20 % respectively.The concentration which inhibited half colony formation was 18.5 mg·L~(-1).The rate of incorporation of -TdR into DNA of L1210 cells induced by 12.0,17.2,24.5,35.0 and 50.0 mg·L~(-1) DADAG for 8 h were 93.6%,83.9%,42.6%,10.1% and 5.7% respectively.Conclusion The inhibitory effect of DADAG on L1210 cells is related to its inhibitory effect on DNA synthesis.