（125）I联合ADM对乳腺癌MCF-7细胞增殖、凋亡的影响

目的研究放射性粒子125I联合化疗药物多柔比星（阿霉素,ADM）对乳腺癌MCF-7细胞增殖、凋亡的影响。方法按2×2析因设计将乳腺癌敏感株MCF-7细胞随机分成A、B、C、D 4组,A组：空白对照组;B组：单纯125I粒子组;C组：单纯ADM组;D组：125I粒子＋ADM组。采用流式细胞术检测各组干预后的细胞周期分布及细胞凋亡率。结果 （1）单纯125I粒子近距离低剂量率持续照射后将MCF-7细胞阻滞于G2~M期1。25I联合ADM将MCF-7细胞周期主要集中在G0~G1期,同时伴有较大比例的细胞凋亡。（2）各组细胞的早期凋亡率分别为：A组（0.99±0.05）%、B组（19.22±4.92）%、C组（16.57±4.73）%、D组（3.16±1.08）%;各组晚期凋亡及坏死率为：A组（0.32±0.18）%、B组（3.16±1.39）%、C组（3.24±0.75）%、D组（28.99±7.96）%,D组晚期凋亡及死亡率明显提高。结论 125I放射性粒子能有效诱导乳腺癌细胞凋亡,与化疗药物ADM联合作用除诱导细胞凋亡,还可导致大量细胞死亡,具有协同、增效的作用。

Effects of radioactive seeds 125I combined with adriamycin on proliferation and apoptosis of breast cancer MCF-7 cells in vitro

Objective To investigate the effects of 125I radioactive seeds combined with adriamycin（ADM） on proliferation and apoptosis of breast cancer MCF-7 cells in vitro.Methods The cultured breast cancer MCF-7 cells were randomly divided into four groups by 2×2 factorial design： 125I group,ADM group,125I combined with ADM group and control group.Cell cycle distribution and apoptosis of MCF-7 cells were analyzed by flow cytometry（FCM）.Results MCF-7 cells were arrested at G2~M phase in 125I group,and arrested at G0~G1 phase with larger proportion of apoptotic cells in 125I combined with ADM group.Flow cytometry and Annexin V staining showed that the earlier period apoptosis rates of MCF-7 cells were（19.22±4.92）% in 125I group,（16.57±4.73）% in ADM group.In 125I with ADM group,the earlier stage apoptosis rate declined to（3.16±1.08）%,however,the rates of advanced stage apoptosis and cells necrosis were raised to（28.99±7.96）%.Conclusions The radioactive seeds125I induces apoptosis of MCF-7 cells.125I combined with ADM causes the cell apoptosis and necrosis,showing synergestic effects on MCF-7 cells.